BMRB: 16582

2J coupling constants in human Ubiquitin

Authors

Schmidt, J.M., Lohr, F.

Assembly

Ubiquitin

Entity

1. Ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Formula weight

8564.731 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

643 J values in 10 lists
Field strength (¹H) 950 MHz, 500 MHz, 600 MHz, 900 MHz, Pressure 1 atm, Temperature 298 K, pH 6.0, 7.0 Detail

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Pressure 1 atm, Temperature 298 K, pH 7.0

Experiment name 3D Cb-coupled [15N,1H]-TROSY-iHNCA, 2D CO-coupled ct-[13C,1H]-HSQC, 3D Cb-coupled [15N,1H]-TROSY-H(N)COCA, 3D Ha-coupled [15N,1H]-TROSY-H(N)COCA, 3D Ha-coupled [15N,1H]-TROSY-iHNCA, 2D Cb-coupled [13C,1H]-HSQC

List #5 2JHaCO, Field strength (¹H) 900 MHz

List #6 2JHaCb, Field strength (¹H) 950 MHz

List #7 2JCbCO, Field strength (¹H) 500 MHz

List #8 2JN_Ha, Field strength (¹H) 500 MHz

List #9 2JN_Cb, Field strength (¹H) 500 MHz

Pressure 1 atm, Temperature 298 K, pH 6.0

Experiment name 3D CO-coupled ct-[15N,1H]-TROSY-HNCA, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY

List #1 2JCaN_, Field strength (¹H) 600 MHz

List #2 2JHNCa, Field strength (¹H) 600 MHz

List #3 2JCOHN, Field strength (¹H) 600 MHz

List #4 2JCOCa, Field strength (¹H) 500 MHz

List #10 2JN_CO, Field strength (¹H) 600 MHz

Release date

2010-02-07

Citation 1

Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract

Show more 5 citaions

Citation 2

Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C
Vuister, G.W., Bax, A.
J. Biomol. NMR (1992), 2, 401-405, PubMed 1511238 , Abstract

Citation 3

[13C]-constant-time [15N,1H]-TROSY-HNCA for sequential assignment of large proteins
Salzmann, M., Pervushin, K., Wider, G., Senn, H., Wuthrich, K.
J. Biomol. NMR (1999), 14, 85-88

Citation 4

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 5

Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract

Citation 6

Determination of spin-spin couplings from ultrahigh resolution 3D NMR spectra obtained by optimized random sampling and multidimensional Fourier transformation
Kazimierczuk, K., Zawadzka, A., Kozminski, W., Zhukov, I.
J. Am. Chem. Soc. (2008), 130, 5404-5405, PubMed 18376830 , DOI 10.1021/ja800622p , Abstract

Hide non-primary 5 citaions

Related entities 1. Ubiquitin, : 1 : 292 : 11 : 72 : 321 entities Detail

Interaction partners 1. Ubiquitin, : 27 interactors Detail

Experiments performed 10 experiments Detail