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Found 1 Document (0.367 seconds)

BMRB: 16583

1QH7

2J coupling constants in Xylanase from Bacillus agaradhaerens

Authors: Schmidt, J.M., Lohr, F.
Assembly: Xylanase
Entity: 1. Xylanase (polymer, Thiol state: all free), 207 monomers, 23154.44 Da Detail
MIVTDNSIGN HDGYDYEFWK DSGGSGTMIL NHGGTFSAQW NNVNNILFRK GKKFNETQTH QQVGNMSINY GANFQPNGNA YLCVYGWTVD PLVEYYIVDS WGNWRPPGAT PKGTITVDGG TYDIYETLRV NQPSIKGIAT FKQYWSVRRS KRTSGTISVS NHFRAWENLG MNMGKMYEVA LTVEGYQSSG SANVYSNTLR INGNPLS
Formula weight: 23154.44 Da
Source organism: Salipaludibacillus agaradhaerens
Exptl. method: solution NMR
Data set: coupling_constants
Coupling constant: 977 J values in 6 lists
Field strength (¹H) 950 MHz, 900 MHz, Pressure 1 atm, Temperature 302 K, pH 5.4 Detail
Pressure 1 atm, Temperature 302 K, pH 5.4
Experiment name 2D CO-coupled ct-[13C,1H]-HSQC, 3D quantitative J-correlated [15N,1H]-TROSY-HNC, 3D Ca-coupled [15N,1H]-TROSY-HNCO, 2D IPAP-type HN(a/b-NCO-J)-TROSY, 3D Ha-coupled [15N,1H]-TROSY-iHNCA, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 2D Cb-coupled [13C,1H]-HSQC
List #1 2JCaN_, Field strength (¹H) 950 MHz
List #2 2JHNCa, Field strength (¹H) 950 MHz
List #3 2JCOHN, Field strength (¹H) 950 MHz
List #4 2JN_Ha, Field strength (¹H) 950 MHz
List #5 2JHaCO, Field strength (¹H) 900 MHz
List #6 2JHaCb, Field strength (¹H) 950 MHz
Release date: 2010-02-07
Citation 1: Correlation of (2)J couplings with protein secondary structure
Schmidt, J.M., Hua, Y., Lohr, F.
Proteins (2010), 78, 1544-1562, PubMed 20131375 , DOI 10.1002/prot.22672 , Abstract
Citation 2: Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C
Vuister, G.W., Bax, A.
J. Biomol. NMR (1992), 2, 401-405, PubMed 1511238 , DOI 10.1007/bf01874818 , Abstract
Citation 3: Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract
Citation 4: Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment
Permi, P.
J. Biomol. NMR (2003), 27, 341-349, PubMed 14512731 , Abstract
Citation 5: Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment
Wienk, H.L.J., Martinez, M.M., Yalloway, G.N., Schmidt, J.M., Perez, C., Ruterjans, H., Lohr, F.
J. Biomol. NMR (2003), 25, 133-145, PubMed 12652122 , Abstract

Related entities 1. Xylanase, : 1 : 4 : 160 entities Detail

Experiments performed 7 experiments Detail

1 3D Ca-coupled [15N,1H]-TROSY-HNCO

Instrument

Bruker DRX - 500 MHz 1H{13C,15N}-triple-resonance three-axis PFG probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

2 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY

Instrument

Bruker Avance - 950 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

3 2D IPAP-type HN(a/b-NCO-J)-TROSY

Instrument

Bruker Avance - 950 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

4 3D Ha-coupled [15N,1H]-TROSY-iHNCA

Instrument

Bruker Avance - 950 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

5 3D quantitative J-correlated [15N,1H]-TROSY-HNC

Instrument

Bruker Avance - 800 MHz room-temperature and cryogenic triple-resonance z-gradient probes

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

6 2D Cb-coupled [13C,1H]-HSQC

Instrument

Bruker Avance - 950 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

7 2D CO-coupled ct-[13C,1H]-HSQC

Instrument

Bruker Avance - 900 MHz cryogenic triple-resonance z-gradient probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 302 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	Xylanase	[U-95% 13C; U-95% 15N]	1.5 mM
2	sodium acetate	natural abundance	10 mM
3	sodium azide	natural abundance	0.03 %
4	Pefabloc protease inhibitor	natural abundance	50 ug/mL
5	D2O	[U-2H]	5 %
6	H2O	natural abundance	95 %

Keywords data mining, statistics, torsion angles, two-bond coupling, 2JHACO measurement, 2JCAN_ measurement, 2JCOHN measurement, 2JHNCA measurement, IPAP method, 2JN_HA measurement, quantitative J correlation

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Found 1 Document (0.367 seconds)

BMRB: 16583

Related entities 1. Xylanase, : 1 : 4 : 160 entities Detail

Experiments performed 7 experiments Detail

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