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BMRB: 16600

Backbone resonance assignments of cgGFP

Authors

Feng, Y., Wang, J.

Assembly

cgGFP

Entity

1. cgGFP (polymer, Thiol state: all free), 235 monomers, 26367.46 Da Detail

MTALTEGAKL FEKEIPYITE LEGDVEGMKF IIKGEGTGDA TTGTIKAKYI CTTGDLPVPW ATILSSLSYG VFCFAKYPRH IADFFKSTQP DGYSQDRIIS FDNDGQYDVK AKVTYENGTL YNRVTVKGTG FKSNGNILGM RVLYHSPPHA VYILPDRKNG GMKIEYNKAF DVMGGGHQMA RHAQFNKPLG AWEEDYPLYH HLTVWTSFGK DPDDDETDHL TIVEVIKAVD LETYR

Formula weight

26367.46 Da

Source organism

Clytia gregaria

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 46.8 %, Completeness (bb): 86.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.8 % (1278 of 2733)	30.6 % (432 of 1410)	58.7 % (636 of 1083)	87.5 % (210 of 240)
Backbone	86.2 % (1197 of 1388)	76.1 % (367 of 482)	90.9 % (620 of 682)	93.8 % (210 of 224)
Sidechain	18.0 % (281 of 1557)	7.1 % (66 of 928)	35.1 % (215 of 613)	0.0 % (0 of 16)
Aromatic	0.7 % (2 of 290)	1.4 % (2 of 145)	0.0 % (0 of 142)	0.0 % (0 of 3)
Methyl	9.4 % (23 of 244)	4.1 % (5 of 122)	14.8 % (18 of 122)

1. gfp

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	gfp	[U-13C; U-15N; U-2H]	0.8 mM
2	Tris-HCl	natural abundance	20 mM
3	sodium chloride	natural abundance	10 mM
4	EDTA	natural abundance	2 mM
5	DSS	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	gfp	[U-13C; U-15N]	0.8 mM
9	Tris-HCl	natural abundance	20 mM
10	sodium chloride	natural abundance	10 mM
11	EDTA	natural abundance	2 mM
12	DSS	natural abundance	0.01 %
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.62 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.62 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.7 ppm, Outliers: 1 Detail

Release date

2010-09-27

Citation

NMR-derived topology of a GFP-photoprotein energy transfer complex
Titushin, M.S., Feng, Y., Stepanyuk, G.A., Li, Y., Markova, S.V., Golz, S., Wang, B., Lee, J., Wang, J., Vysotski, E.S., Liu, Z.
J. Biol. Chem. (2010), 285, 40891-40900, PubMed 20926380 , DOI 10.1074/jbc.M110.133843 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16599 released on 2010-09-27
Title Backbone resonance assignments of clytin

Related entities 1. cgGFP, : 102 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC