BMRB: 16602

1H, 13C and 15N Chemical Shift Assignment Assignment of Human Membrane Protein KCNE3 in LMPC micelles

Authors

Kang, C., Vanoye, C.G., Sakakura, M.C., Welch, R.C., Sanders, C.R.

Assembly

KCNE3

Entity

1. KCNE3 (polymer, Thiol state: all free), 112 monomers, 12778.40 Da Detail

MGHHHHHHGM ETTNGTETWY ESLHAVLKAL NATLHSNLLC RPGPGLGPDN QTEERRASLP GRDDNSYMYI LFVMFLFAVT VGSLILGYTR SRKVDKRSDP YHVYIKNRVS MI

Formula weight

12778.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 36.3 %, Completeness (bb): 68.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	36.3 % (472 of 1300)	17.3 % (116 of 672)	51.5 % (264 of 513)	80.0 % (92 of 115)
Backbone	68.1 % (451 of 662)	42.1 % (96 of 228)	80.7 % (264 of 327)	85.0 % (91 of 107)
Sidechain	14.6 % (108 of 741)	4.5 % (20 of 444)	30.1 % (87 of 289)	12.5 % (1 of 8)
Aromatic	2.4 % (3 of 126)	3.2 % (2 of 63)	0.0 % (0 of 62)	100.0 % (1 of 1)
Methyl	9.3 % (11 of 118)	10.2 % (6 of 59)	8.5 % (5 of 59)

1. KCNE3

MGHHHHHHGM ETTNGTETWY ESLHAVLKAL NATLHSNLLC RPGPGLGPDN QTEERRASLP GRDDNSYMYI LFVMFLFAVT VGSLILGYTR SRKVDKRSDP YHVYIKNRVS MI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 6.5, Details 2H, 13C and 15N labeled in LMPC micelles.

#	Name	Isotope labeling	Type	Concentration
1	D2O	natural abundance		10 %
2	DTT	natural abundance		2 mM
3	EDTA	natural abundance		2 mM
4	imidazole	natural abundance		250 mM
5	LMPC	natural abundance		10 %
6	KCNE3	[U-100% 13C; U-100% 15N; U-80% 2H]		0.8 mM

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LACS Plot; CA

Referencing offset: 0.3 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.3 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.83 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Release date

2010-02-10

Citation

Functional delivery of a membrane protein into oocyte membranes using bicelles
Kang, C., Vanoye, C.G., Welch, R.C., Van Horn, W.D., Sanders, C.R.
Biochemistry (2010), 49, 653-655, PubMed 20044833 , DOI 10.1021/bi902155t , Abstract

Related entities 1. KCNE3, : 2 : 5 entities Detail

Interaction partners 1. KCNE3, : 10 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail