Phosphorylation of SUMO-interacting motif by CK2 enhances Daxx SUMO binding activity.
MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.9 % (1296 of 1365) | 94.7 % (681 of 719) | 94.5 % (494 of 523) | 98.4 % (121 of 123) |
Backbone | 97.8 % (679 of 694) | 97.5 % (232 of 238) | 97.7 % (335 of 343) | 99.1 % (112 of 113) |
Sidechain | 93.1 % (726 of 780) | 93.3 % (449 of 481) | 92.7 % (268 of 289) | 90.0 % (9 of 10) |
Aromatic | 57.6 % (38 of 66) | 57.6 % (19 of 33) | 57.6 % (19 of 33) | |
Methyl | 96.3 % (104 of 108) | 96.3 % (52 of 54) | 96.3 % (52 of 54) |
1. SUMO1
MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGG2. DAXX20
KTSVATQCDP EEIIVLSDSDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SUMO1 | [U-100% 15N] | 0.27 ~ 0.5 mM | |
2 | DAXX20 | natural abundance | 0.0 ~ 1.7 mM | |
3 | potassium phosphate | natural abundance | 10 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | DTT | natural abundance | 2 mM | |
6 | EDTA | natural abundance | 0.1 mM | |
7 | sodium azide | natural abundance | 0.001 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses synthetic DAXX20
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | SUMO1 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
11 | DAXX20 | natural abundance | 2 mM | |
12 | potassium phosphate | natural abundance | 10 mM | |
13 | potassium chloride | natural abundance | 100 mM | |
14 | DTT | natural abundance | 2 mM | |
15 | EDTA | natural abundance | 0.1 mM | |
16 | sodium azide | natural abundance | 0.001 % | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | SUMO1 | natural abundance | 2 mM | |
20 | DAXX20 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
21 | potassium phosphate | natural abundance | 10 mM | |
22 | potassium chloride | natural abundance | 100 mM | |
23 | DTT | natural abundance | 2 mM | |
24 | EDTA | natural abundance | 0.1 mM | |
25 | sodium azide | natural abundance | 0.001 % | |
26 | H2O | natural abundance | 90 % | |
27 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 290 (±0.1) K, pH 6.5 (±0.05), Details Uses Recombinant DAXX20
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
28 | SUMO1 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
29 | DAXX20 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
30 | potassium phosphate | natural abundance | 10 mM | |
31 | potassium chloride | natural abundance | 100 mM | |
32 | DTT | natural abundance | 2 mM | |
33 | EDTA | natural abundance | 0.1 mM | |
34 | sodium azide | natural abundance | 0.001 % | |
35 | Pf1 phage | natural abundance | 0.0066 w/v | |
36 | H2O | natural abundance | 90 % | |
37 | D2O | natural abundance | 10 % |