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1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity
Authors
Yao, S., Murphy, J.M., Low, A., Norton, R.S.
Assembly
a single polypeptide chain
Entity
1. a single polypeptide chain (polymer, Thiol state: all disulfide bound), 127 monomers, 14286.07 Da Detail

GAMDTHRLTR TLNCSSIVKE IIGKLPEPEL KTDDEGPSLR NKSFRRVNLS KFVESQGEVD PEDRYVIKSN LQKLNACLPT SANDSALPGV FIRDLDDFRK KLRFYMVHLN DLETVLTSRP PQPASGS


Formula weight
14286.07 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.6 %, Completeness: 85.3 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All85.3 % (1266 of 1484)86.4 % (675 of 781)81.7 % (470 of 575)94.5 % (121 of 128)
Backbone95.3 % (709 of 744)96.4 % (242 of 251)93.6 % (351 of 375)98.3 % (116 of 118)
Sidechain77.8 % (670 of 861)81.7 % (433 of 530)72.3 % (232 of 321)50.0 % (5 of 10)
Aromatic36.5 % (27 of 74)73.0 % (27 of 37) 0.0 % (0 of 37)
Methyl89.7 % (122 of 136)92.6 % (63 of 68)86.8 % (59 of 68)

1. mIL3

GAMDTHRLTR TLNCSSIVKE IIGKLPEPEL KTDDEGPSLR NKSFRRVNLS KFVESQGEVD PEDRYVIKSN LQKLNACLPT SANDSALPGV FIRDLDDFRK KLRFYMVHLN DLETVLTSRP PQPASGS

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 6.7, Details 20 mM potassium phosphate, and 0.02% (wv) sodium azide at pH 6.7


#NameIsotope labelingTypeConcentration
1mIL3[U-100% 15N]0.28 mM
2potassium phosphatenatural abundance20 mM
3sodium azidenatural abundance0.2 %
4H2Onatural abundance95 %
5D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 6.7, Details 20 mM potassium phosphate, and 0.02% (wv) sodium azide at pH 6.7


#NameIsotope labelingTypeConcentration
6mIL3[U-100% 13C; U-100% 15N]0.28 mM
7potassium phosphatenatural abundance20 mM
8sodium azidenatural abundance0.2 %
9H2Onatural abundance95 %
10D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.35 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.35 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.05 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: -0.23 ppm, Outliers: 1 Detail
Release date
2010-05-04
Citation
1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity
Yao, S., Murphy, J.M., Low, A., Norton, R.S.
Biomol. NMR Assign. (2010), 4, 73-77, PubMed 20174897 , DOI 10.1007/s12104-010-9213-1 ,
Related entities 1. a single polypeptide chain, : 1 : 2 : 9 entities Detail
Experiments performed 11 experiments Detail
Chemical shift validation 3 contents Detail