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BMRB: 16614

2L3O

1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity

Authors

Yao, S., Murphy, J.M., Low, A., Norton, R.S.

Assembly

a single polypeptide chain

Entity

1. a single polypeptide chain (polymer, Thiol state: all disulfide bound), 127 monomers, 14286.07 Da Detail

GAMDTHRLTR TLNCSSIVKE IIGKLPEPEL KTDDEGPSLR NKSFRRVNLS KFVESQGEVD PEDRYVIKSN LQKLNACLPT SANDSALPGV FIRDLDDFRK KLRFYMVHLN DLETVLTSRP PQPASGS

Formula weight

14286.07 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 85.3 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.3 % (1266 of 1484)	86.4 % (675 of 781)	81.7 % (470 of 575)	94.5 % (121 of 128)
Backbone	95.3 % (709 of 744)	96.4 % (242 of 251)	93.6 % (351 of 375)	98.3 % (116 of 118)
Sidechain	77.8 % (670 of 861)	81.7 % (433 of 530)	72.3 % (232 of 321)	50.0 % (5 of 10)
Aromatic	36.5 % (27 of 74)	73.0 % (27 of 37)	0.0 % (0 of 37)
Methyl	89.7 % (122 of 136)	92.6 % (63 of 68)	86.8 % (59 of 68)

1. mIL3

GAMDTHRLTR TLNCSSIVKE IIGKLPEPEL KTDDEGPSLR NKSFRRVNLS KFVESQGEVD PEDRYVIKSN LQKLNACLPT SANDSALPGV FIRDLDDFRK KLRFYMVHLN DLETVLTSRP PQPASGS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 6.7, Details 20 mM potassium phosphate, and 0.02% (wv) sodium azide at pH 6.7

#	Name	Isotope labeling	Concentration
1	mIL3	[U-100% 15N]	0.28 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium azide	natural abundance	0.2 %
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 K, pH 6.7, Details 20 mM potassium phosphate, and 0.02% (wv) sodium azide at pH 6.7

#	Name	Isotope labeling	Concentration
6	mIL3	[U-100% 13C; U-100% 15N]	0.28 mM
7	potassium phosphate	natural abundance	20 mM
8	sodium azide	natural abundance	0.2 %
9	H2O	natural abundance	95 %
10	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.9445 ppm	na	indirect	0.2514495
¹H	water	protons	4.9445 ppm	internal	direct	1.0
¹⁵N	water	protons	4.9445 ppm	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.9445 ppm	na	indirect	0.2514495
¹H	water	protons	4.9445 ppm	internal	direct	1.0
¹⁵N	water	protons	4.9445 ppm	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.9445 ppm	na	indirect	0.2514495
¹H	water	protons	4.9445 ppm	internal	direct	1.0
¹⁵N	water	protons	4.9445 ppm	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.9445 ppm	na	indirect	0.2514495
¹H	water	protons	4.9445 ppm	internal	direct	1.0
¹⁵N	water	protons	4.9445 ppm	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

Release date

2010-05-04

Citation

1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity
Yao, S., Murphy, J.M., Low, A., Norton, R.S.
Biomol. NMR Assign. (2010), 4, 73-77, PubMed 20174897 , DOI 10.1007/s12104-010-9213-1 , Abstract

Related entities 1. a single polypeptide chain, : 1 : 2 : 9 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC