NMR structure of the J-domain (residues 2-72) in the Escherichia coli CbpA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.5 % (806 of 881) | 90.8 % (422 of 465) | 92.1 % (314 of 341) | 93.3 % (70 of 75) |
Backbone | 97.2 % (420 of 432) | 96.6 % (140 of 145) | 97.7 % (212 of 217) | 97.1 % (68 of 70) |
Sidechain | 87.9 % (457 of 520) | 88.1 % (282 of 320) | 88.7 % (173 of 195) | 40.0 % (2 of 5) |
Aromatic | 82.9 % (68 of 82) | 97.6 % (40 of 41) | 66.7 % (26 of 39) | 100.0 % (2 of 2) |
Methyl | 98.4 % (61 of 62) | 96.8 % (30 of 31) | 100.0 % (31 of 31) |
1. J-domain of CbpA
GSELKDYYAI MGVKPTDDLK TIKTAYRRLA RKYHPDVSKE PDAEARFKEV AEAWEVLSDE QRRAEYDQMW QHRSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | J-domain of CbpA | [U-13C; U-15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | sodium chloride | natural abundance | 250 mM | |
4 | DTT | natural abundance | 1 mM |