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BMRB: 16628

2KXL

Sequence-specific 1H, 13C and 15N resonance assignments of the nucleotide-free wildtype cyclic nucleotide binding domain from a cyclic nucleotide-activated K+ channel

Authors

Schuenke, S., Lecher, J., Stoldt, M., Willbold, D.

Assembly

MloK1 CNBD monomer

Entity

1. MloK1 CNBD monomer (polymer, Thiol state: not present), 142 monomers, 14968.09 Da Detail

GSQEVRRGDF VRNWQLVAAV PLFQKLGPAV LVEIVRALRA RTVPAGAVIC RIGEPGDRMF FVVEGSVSVA TPNPVELGPG AFFGEMALIS GEPRSATVSA ATTVSLLSLH SADFQMLCSS SPEIAEIFRK TALERRGAAA SA

Formula weight

14968.09 Da

Source organism

Mesorhizobium loti

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 90.9 %, Completeness (bb): 93.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.9 % (1421 of 1563)	92.3 % (740 of 802)	87.9 % (546 of 621)	96.4 % (135 of 140)
Backbone	93.3 % (778 of 834)	96.9 % (278 of 287)	89.9 % (372 of 414)	96.2 % (128 of 133)
Sidechain	89.5 % (769 of 859)	89.7 % (462 of 515)	89.0 % (300 of 337)	100.0 % (7 of 7)
Aromatic	39.6 % (38 of 96)	39.6 % (19 of 48)	38.3 % (18 of 47)	100.0 % (1 of 1)
Methyl	97.9 % (184 of 188)	97.9 % (92 of 94)	97.9 % (92 of 94)

1. MloK1 CNBD

GSQEVRRGDF VRNWQLVAAV PLFQKLGPAV LVEIVRALRA RTVPAGAVIC RIGEPGDRMF FVVEGSVSVA TPNPVELGPG AFFGEMALIS GEPRSATVSA ATTVSLLSLH SADFQMLCSS SPEIAEIFRK TALERRGAAA SA

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	MloK1 CNBD	[U-15N]	0.5 mM
2	TRIS	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.02 %
5	D2O	[U-100% 2H]	5 %
6	H2O	natural abundance	95 %
7	EDTA	natural abundance	200 uM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	MloK1 CNBD	[U-13C; U-15N]	0.5 mM
9	TRIS	[U-99% 2H]	10 mM
10	sodium chloride	natural abundance	100 mM
11	sodium azide	natural abundance	0.02 %
12	D2O	[U-100% 2H]	5 %
13	H2O	natural abundance	95 %
14	EDTA	natural abundance	200 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Release date

2010-07-18

Citation

Resonance assignments of the nucleotide-free wildtype MloK1 cyclic nucleotide-binding domain
Schunke, S., Lecher, J., Stoldt, M., Kaupp, U., Willbold, D.
Biomol. NMR Assign. (2010), 4, 147-150, PubMed 20449776 , DOI 10.1007/s12104-010-9231-z , Abstract

Related entities 1. MloK1 CNBD monomer, : 1 : 1 : 20 : 170 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC