Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase
GIKQYSQEEL KEMALVEIAH ELFEEHKKPV PFQELLNEIA SLLGVKKEEL GDRIAQFYTD LNIDGRFLAL SDQTWGLRSW YPYDQLDEET QLEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.2 % (1167 of 1213) | 96.1 % (608 of 633) | 96.2 % (455 of 473) | 97.2 % (104 of 107) |
Backbone | 95.9 % (564 of 588) | 95.0 % (190 of 200) | 96.2 % (281 of 292) | 96.9 % (93 of 96) |
Sidechain | 96.7 % (695 of 719) | 96.5 % (418 of 433) | 96.7 % (266 of 275) | 100.0 % (11 of 11) |
Aromatic | 90.6 % (116 of 128) | 90.6 % (58 of 64) | 90.3 % (56 of 62) | 100.0 % (2 of 2) |
Methyl | 100.0 % (104 of 104) | 100.0 % (52 of 52) | 100.0 % (52 of 52) |
1. N delta
GIKQYSQEEL KEMALVEIAH ELFEEHKKPV PFQELLNEIA SLLGVKKEEL GDRIAQFYTD LNIDGRFLAL SDQTWGLRSW YPYDQLDEET QLEHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N_delta | 0.0 ~ 0.0 mM | ||
2 | sodium chloride | natural abundance | 10 mM | |
3 | sodium azide | natural abundance | 50 uM | |
4 | phosphate buffer | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | N_delta | 0.0 ~ 0.0 mM | ||
6 | sodium chloride | natural abundance | 10 mM | |
7 | sodium azide | natural abundance | 50 uM | |
8 | phosphate buffer | natural abundance | 20 mM | |
9 | filamentous Pf1 bacteriophage | natural abundance | 14.29 mg/mL |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | N_delta | 0.0 ~ 0.0 mM | ||
11 | sodium chloride | natural abundance | 10 mM | |
12 | sodium azide | natural abundance | 50 uM | |
13 | phosphate buffer | natural abundance | 20 mM | |
14 | polyacrylamide gel | natural abundance | 5 % |