Heterodimeric association of Transmembrane domains of ErbB1 and ErbB2 receptors Enabling Kinase Activation
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.3 % (945 of 1035) | 95.0 % (512 of 539) | 86.6 % (354 of 409) | 90.8 % (79 of 87) |
Backbone | 89.4 % (463 of 518) | 97.8 % (177 of 181) | 81.5 % (207 of 254) | 95.2 % (79 of 83) |
Sidechain | 93.9 % (559 of 595) | 93.6 % (335 of 358) | 96.1 % (224 of 233) | 0.0 % (0 of 4) |
Aromatic | 75.0 % (18 of 24) | 100.0 % (12 of 12) | 50.0 % (6 of 12) | |
Methyl | 97.3 % (146 of 150) | 97.3 % (73 of 75) | 97.3 % (73 of 75) |
1. ErbB1TM
EGCPTNGPKI PSIATGMVGA LLLLLVVALG IGLFMRRRHI VRKR2. ErbB2TM
GCPAEQRASP LTSIISAVVG ILLVVVLGVV FGILIKRRQQ KIRKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 4.5, Details N15 labeled protein in bicelles
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ErbB1TM | [U-100% 15N] | 1 mM | |
2 | ErbB2TM | [U-100% 15N] | 1 mM | |
3 | DMPC | natural abundance | 12 mM | |
4 | DHPC | natural abundance | 48 mM | |
5 | EDTA | natural abundance | 3 mM | |
6 | TCEP | natural abundance | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 4.5, Details mixture of labeled ErbB1 and unlabeled ErbB2 protein in bicelles
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | ErbB1TM | [U-100% 13C; U-100% 15N] | 1 mM | |
10 | ErbB2TM | natural abundance | 1 mM | |
11 | DMPC | [U-2H] | 12 mM | |
12 | DHPC | [U-2H] | 48 mM | |
13 | EDTA | natural abundance | 3 mM | |
14 | TCEP | natural abundance | 1 mM | |
15 | D2O | natural abundance | 100 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 4.5, Details mixture of labeled ErbB2 and unlabeled ErbB1 protein in bicelles in water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | ErbB1TM | natural abundance | 1 mM | |
17 | ErbB2TM | [U-100% 13C; U-100% 15N] | 1 mM | |
18 | DMPC | [U-2H] | 12 mM | |
19 | DHPC | [U-2H] | 48 mM | |
20 | EDTA | natural abundance | 3 mM | |
21 | TCEP | natural abundance | 1 mM | |
22 | H2O | natural abundance | 95 % | |
23 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 4.5, Details mixture of labeled ErbB1 and unlabeled ErbB2 protein in bicelles in water
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
24 | ErbB1TM | [U-100% 13C; U-100% 15N] | 1 mM | |
25 | ErbB2TM | natural abundance | 1 mM | |
26 | DMPC | [U-2H] | 12 mM | |
27 | DHPC | [U-2H] | 48 mM | |
28 | EDTA | natural abundance | 3 mM | |
29 | TCEP | natural abundance | 1 mM | |
30 | H2O | natural abundance | 95 % | |
31 | D2O | natural abundance | 5 % |