Sequence-specific 1H, 15N and 13C resonance assignments of Sugarcane defensin 5 (Sd5).
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS9:SG | 1:CYS57:SG |
2 | disulfide | sing | 1:CYS26:SG | 1:CYS51:SG |
3 | disulfide | sing | 1:CYS30:SG | 1:CYS53:SG |
4 | disulfide | sing | 1:CYS20:SG | 1:CYS42:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.4 % (733 of 811) | 92.6 % (398 of 430) | 86.5 % (270 of 312) | 94.2 % (65 of 69) |
Backbone | 94.2 % (388 of 412) | 96.4 % (135 of 140) | 92.3 % (192 of 208) | 95.3 % (61 of 64) |
Sidechain | 88.0 % (409 of 465) | 90.7 % (263 of 290) | 83.5 % (142 of 170) | 80.0 % (4 of 5) |
Aromatic | 68.2 % (45 of 66) | 81.8 % (27 of 33) | 56.3 % (18 of 32) | 0.0 % (0 of 1) |
Methyl | 95.2 % (40 of 42) | 100.0 % (21 of 21) | 90.5 % (19 of 21) |
1. Sd5- Sugarcane defensin 5
HTPTPTPICK SRSHEYKGRC IQDMDCNAAC VKESESYTGG FCNGRPPFKQ CFCTKPCKRE RAAATLRWPG LSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sd5- Sugarcane defensin 5 | [U-99% 15N] | 1 mM | |
2 | sodium phophate buffer | natural abundance | 5 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Sd5- Sugarcane defensin 5 | [U-99% 13C; U-99% 15N] | 0.7 mM | |
6 | sodium phophate buffer | natural abundance | 5 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |