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Structure of the insecticidal toxin TaITX-1
Authors
Mobli, M., King, G.F.
Assembly
TaITX-1
Entity
1. TaITX-1 (polymer, Thiol state: all disulfide bound), 51 monomers, 5773.323 Da Detail

SEPDEICRAR MTHKEFNYKS NVCNGCGDQV AACEAECFRN DVYTACHEAQ K


Formula weight
5773.323 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS7:SG1:CYS37:SG
2disulfidesing1:CYS23:SG1:CYS33:SG
3disulfidesing1:CYS26:SG1:CYS46:SG

Source organism
Eratigena agrestis
Exptl. method
solution NMR
Refine. method
TORSION ANGLE DYNAMICS
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All93.0 % (519 of 558)96.2 % (280 of 291)87.2 % (184 of 211)98.2 % (55 of 56)
Backbone96.7 % (294 of 304)97.1 % (100 of 103)96.7 % (146 of 151)96.0 % (48 of 50)
Sidechain87.8 % (266 of 303)94.1 % (177 of 188)76.1 % (83 of 109)100.0 % (6 of 6)
Aromatic43.2 % (19 of 44)86.4 % (19 of 22) 0.0 % (0 of 22)
Methyl93.8 % (30 of 32)93.8 % (15 of 16)93.8 % (15 of 16)

1. Ta1a

SEPDEICRAR MTHKEFNYKS NVCNGCGDQV AACEAECFRN DVYTACHEAQ K

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.4


#NameIsotope labelingTypeConcentration
1Ta1a[U-100% 13C; U-100% 15N]150 uM
2H2Onatural abundance95 %
3D2Onatural abundance5 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 4.4


#NameIsotope labelingTypeConcentration
4Ta1a[U-100% 13C; U-100% 15N]150 uM
5D2Onatural abundance100 %

Protein Blocks Logo
Calculated from 25 models in PDB: 2KSL, Strand ID: A Detail


Release date
2015-06-18
Citation
Weaponization of a Hormone: Convergent Recruitment of Hyperglycemic Hormone into the Venom of Arthropod Predators
Mobli, M., Grimm, L.L., Low, C., Volker, H., Bryan, F.G., King, G.F.
Structure (2015), 23, 1283-1292, PubMed 26073605 , DOI 10.1016/j.str.2015.05.003 ,
Related entities 1. TaITX-1, : 1 : 1 : 1 : 5 entities Detail
Experiments performed 7 experiments Detail
nullKeywords crustacean hyperglycemic hormone, insecticidal toxin, molt-inhibiting hormone, neurotoxin, spider toxin