Backbone 1H, 13C, and 15N Chemical Shift Assignments for Oryctin
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.8 % (628 of 749) | 82.4 % (324 of 393) | 82.5 % (241 of 292) | 98.4 % (63 of 64) |
Backbone | 96.1 % (367 of 382) | 98.5 % (128 of 130) | 93.3 % (180 of 193) | 100.0 % (59 of 59) |
Sidechain | 74.8 % (320 of 428) | 74.5 % (196 of 263) | 75.0 % (120 of 160) | 80.0 % (4 of 5) |
Aromatic | 16.7 % (7 of 42) | 33.3 % (7 of 21) | 0.0 % (0 of 21) | |
Methyl | 80.6 % (50 of 62) | 93.5 % (29 of 31) | 67.7 % (21 of 31) |
1. Oryctin
VPVGSDCEPK LCTMDLVPHC FLNPEKGIVV VHGGCALSKY KCQNPNHEKL GYTHECEEAI KNAPRPSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 13C,15N-labeled oryctin was dissolved into 50 mM sodium phosphate buffer (pH 6.8), 100 mM NaCl, 0.02% NaN3 in 90% H2O/10% D2O (v/v) to a final concentration of 2 mM.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | oryctin | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium chloride | natural abundance | 100 mM | |
3 | D2O | natural abundance | 10 % | |
4 | H2O | natural abundance | 90 % | |
5 | sodium phosphate | natural abundance | 50 mM | |
6 | sodium azide | natural abundance | 0.02 % w/v |