BMRB: 16679

1H, 13C and 15N assignment of the C terminal domain of GNA2132 from Neisseria meningitidis

Authors

Esposito, V., Veggi, D., Musi, V., Pizza, M., Pastore, A.

Assembly

GNA 2132

Entity

1. GNA 2132 (polymer, Thiol state: not present), 193 monomers, 20609.51 Da Detail

MASLPAEMPL IPVNQADTLI VDGEAVSLTG HSGNIFAPEG NYRYLTYGAE KLPGGSYALR VQGEPAKGEM LAGTAVYNGE VLHFHTENGR PYPTRGRFAA KVDFGSKSVD GIIDSGDDLH MGTQKFKAAI DGNGFKGTWT ENGGGDVSGR FYGPAGEEVA GKYSYRPTDA EKGGFGVFAG KKEQDLEHHH HHH

Formula weight

20609.51 Da

Source organism

Neisseria meningitidis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.8 %, Completeness: 73.3 %, Completeness (bb): 78.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.3 % (1552 of 2118)	74.4 % (813 of 1093)	70.6 % (586 of 830)	78.5 % (153 of 195)
Backbone	78.2 % (890 of 1138)	79.9 % (325 of 407)	76.5 % (419 of 548)	79.8 % (146 of 183)
Sidechain	68.9 % (787 of 1142)	71.1 % (488 of 686)	65.8 % (292 of 444)	58.3 % (7 of 12)
Aromatic	67.3 % (144 of 214)	75.7 % (81 of 107)	58.5 % (62 of 106)	100.0 % (1 of 1)
Methyl	64.9 % (109 of 168)	67.9 % (57 of 84)	61.9 % (52 of 84)

1. GNA 2132

MASLPAEMPL IPVNQADTLI VDGEAVSLTG HSGNIFAPEG NYRYLTYGAE KLPGGSYALR VQGEPAKGEM LAGTAVYNGE VLHFHTENGR PYPTRGRFAA KVDFGSKSVD GIIDSGDDLH MGTQKFKAAI DGNGFKGTWT ENGGGDVSGR FYGPAGEEVA GKYSYRPTDA EKGGFGVFAG KKEQDLEHHH HHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7, Details GNA2132 0.5 mM uniformelly 13C and 15N labelled, 20 mM tris HCl, 50 mM sodium chloride, 30 mM dodecylphosphocoline

#	Name	Isotope labeling	Type	Concentration
1	GNA 2132	[U-100% 13C; U-100% 15N]		0.5 mM
2	TRIS	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	DPC	natural abundance		30 mM

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.695 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.695 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.695 ppm	internal	direct	1.0

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LACS Plot; CO

Referencing offset: 0.87 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.695 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 10 models in PDB: 2LFU, Strand ID: A Detail

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Release date

2010-04-07

Citation

1H, 13C and 15N assignment of the C-terminal domain of GNA2132 from Neisseria meningitidis
Esposito, V., Musi, V., Veggi, D., Pastore, A., Pizza, M.
Biomol. NMR Assign. (2010), 4, 107-109, PubMed 20300890 , DOI 10.1007/s12104-010-9220-2 , Abstract

Related entities 1. GNA 2132, : 1 : 1 : 10 entities Detail

Experiments performed 10 experiments Detail