Solution structure of human stem cell transcription factor Nanog
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.7 % (1058 of 1083) | 99.3 % (578 of 582) | 99.5 % (396 of 398) | 81.6 % (84 of 103) |
Backbone | 99.6 % (498 of 500) | 100.0 % (166 of 166) | 99.6 % (251 of 252) | 98.8 % (81 of 82) |
Sidechain | 96.6 % (644 of 667) | 99.0 % (412 of 416) | 99.6 % (229 of 230) | 14.3 % (3 of 21) |
Aromatic | 97.1 % (68 of 70) | 100.0 % (35 of 35) | 100.0 % (33 of 33) | 0.0 % (0 of 2) |
Methyl | 100.0 % (70 of 70) | 100.0 % (35 of 35) | 100.0 % (35 of 35) |
1. Nanog
SKQPTSAENS VAKKEDKVPV KKQKTRTVFS STQLCVLNDR FQRQKYLSLQ QMQELSNILN LSYKQVKTWF QNQRMKSKRW QKNNSolvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.7 mM nanog U-15N/13C, 10 mM MOPS, 5 mM Dithiothreitol, 250 mM NaCl
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Nanog | [U-95% 13C; U-90% 15N] | 0.7 mM | |
2 | MOPS | natural abundance | 10 mM | |
3 | Dithiothreitol | natural abundance | 5 mM | |
4 | NaCl | natural abundance | 250 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |