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BMRB: 16687

5YPP

1H, 13C and 15N assignments of the dimeric regulatory subunit (ilvN) of E.coli AHAS I

Authors

Karanth, M.N., Sarma, S.P.

Assembly

ilvN

Entity

1. ilvN (polymer, Thiol state: all free), 98 monomers, 11249.76 × 2 Da Detail

GSMQNTTHDN VILELTVRNH PGVMTHVCGL FARRAFNVEG ILCLPIQDSD KSHIWLLVND DQRLEQMISQ IDKLEDVVKV QRNQSDPTMF NKIAVFFQ

2. VAL (polymer), 1 monomers, 117.146 × 2 Da Detail

V

Total weight

22733.812 Da

Max. entity weight

11249.76 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 86.7 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.7 % (1011 of 1166)	86.8 % (526 of 606)	86.2 % (387 of 449)	88.3 % (98 of 111)
Backbone	93.0 % (541 of 582)	93.4 % (184 of 197)	92.1 % (267 of 290)	94.7 % (90 of 95)
Sidechain	82.9 % (562 of 678)	83.6 % (342 of 409)	83.8 % (212 of 253)	50.0 % (8 of 16)
Aromatic	64.1 % (50 of 78)	69.2 % (27 of 39)	57.9 % (22 of 38)	100.0 % (1 of 1)
Methyl	96.7 % (116 of 120)	96.7 % (58 of 60)	96.7 % (58 of 60)

1. ilvN

GSMQNTTHDN VILELTVRNH PGVMTHVCGL FARRAFNVEG ILCLPIQDSD KSHIWLLVND DQRLEQMISQ IDKLEDVVKV QRNQSDPTMF NKIAVFFQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ilvN	[U-99% 15N]	0.4 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	sodium azide	natural abundance	0.01 %
6	L-Valine	natural abundance	5 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	ilvN	[U-99% 13C; U-99% 15N]	0.4 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	20 mM
12	EDTA	natural abundance	1 mM
13	sodium azide	natural abundance	0.01 %
14	L-Valine	natural abundance	5 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Release date

2010-05-04

Citation

1H, 13C, 15N assignments of the dimeric regulatory subunit (ilvN) of the E. coli AHAS I
Karanth, N., Sarma, S.P.
Biomol. NMR Assign. (2010), 4, 131-133, PubMed 20383786 , DOI 10.1007/s12104-010-9225-x , Abstract

Related entities 1. ilvN, : 1 : 4 : 2 : 10 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC