NMR structure of the PASTA domain 2 and 3 of Mycobacterium tuberculosis of PknB
GHMGPEQREI PDVSTLTYAE AVKKLTAAGF GRFKQANSPS TPELVGKVIG TNPPANQTSA ITNVVIIIVG SGPATKDIPD VAGQTVDVAQ KNLNVYGFTK FSQASVDSPR PAGEVTGTNP PAGTTVPVDS VIELQVSK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.7 % (1254 of 1499) | 93.1 % (718 of 771) | 67.9 % (400 of 589) | 97.8 % (136 of 139) |
Backbone | 96.5 % (774 of 802) | 96.0 % (265 of 276) | 96.5 % (387 of 401) | 97.6 % (122 of 125) |
Sidechain | 73.1 % (601 of 822) | 91.5 % (453 of 495) | 42.8 % (134 of 313) | 100.0 % (14 of 14) |
Aromatic | 45.0 % (27 of 60) | 90.0 % (27 of 30) | 0.0 % (0 of 30) | |
Methyl | 60.3 % (105 of 174) | 98.9 % (86 of 87) | 21.8 % (19 of 87) |
1. PknB PASTA23
GHMGPEQREI PDVSTLTYAE AVKKLTAAGF GRFKQANSPS TPELVGKVIG TNPPANQTSA ITNVVIIIVG SGPATKDIPD VAGQTVDVAQ KNLNVYGFTK FSQASVDSPR PAGEVTGTNP PAGTTVPVDS VIELQVSKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 (±0.1) K, pH 4.6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PknB_PASTA23 | [U-100% 15N] | 0.6 (±0.1) mM | |
2 | sodium acetate | natural abundance | 20 (±0.1) mM | |
3 | H2O | natural abundance | 95 (±0.1) % | |
4 | D2O | natural abundance | 5 (±0.1) % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 283 (±0.1) K, pH 4.6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | PknB_PASTA23 | [U-100% 13C; U-100% 15N] | 0.6 (±0.1) mM | |
6 | sodium acetate | natural abundance | 20 (±0.1) mM | |
7 | H2O | natural abundance | 95 (±0.1) % | |
8 | D2O | natural abundance | 5 (±0.1) % |