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BMRB: 16736

2KTR

NMR STRUCTURE OF P62 PB1 DIMER DETERMINED BASED ON PCS

Authors

Saio, T., Yokochi, M., Kumeta, H., Inagaki, F.

Assembly

P62 PB1 Dimer

Entity

1. P62 PB1 Dimer, entity 1 (polymer, Thiol state: all disulfide bound), 117 monomers, 13058.55 Da Detail

CYVDTNNDGA YEGDELHMGS LTVKAYLLGK EEAAREIRRF SFCFSPEPEA EAAAGPGPSE RLLSRVAVLF PALRPGGFQA HYRAERGDLV AFSSDEELTM AMSYVKDDIF RIYIKEK

2. P62 PB1 Dimer, entity 2 (polymer, Thiol state: not present), 100 monomers, 11144.42 Da Detail

HMSLTVEAYL LGKEEAAREI RRFSFSFSPE PEAEAAAGPG PSERLLSRVA VLFPALRPGG FQAHYRDEDG DLVAFSSDEE LTMAMSYVKD DIFAIYIKEK

3. TB (non-polymer), 158.925 Da

Total weight

24361.895 Da

Max. entity weight

13058.55 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS43:SG

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 29.2 %, Completeness (bb): 61.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	29.2 % (437 of 1495)	19.0 % (244 of 1286)	92.3 % (193 of 209)
Backbone	61.8 % (397 of 642)	46.7 % (204 of 437)	94.1 % (193 of 205)
Sidechain	4.7 % (40 of 853)	4.7 % (40 of 849)	0.0 % (0 of 4)
Aromatic	3.4 % (4 of 118)	3.4 % (4 of 118)
Methyl	6.5 % (7 of 108)	6.5 % (7 of 108)

1. entity 1

CYVDTNNDGA YEGDELHMGS LTVKAYLLGK EEAAREIRRF SFCFSPEPEA EAAAGPGPSE RLLSRVAVLF PALRPGGFQA HYRAERGDLV AFSSDEELTM AMSYVKDDIF RIYIKEK

2. entity 2

HMSLTVEAYL LGKEEAAREI RRFSFSFSPE PEAEAAAGPG PSERLLSRVA VLFPALRPGG FQAHYRDEDG DLVAFSSDEE LTMAMSYVKD DIFAIYIKEK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-99% 13C; U-99% 15N]	0.5 mM
2	entity_2	natural abundance	0.5 mM
3	MES	natural abundance	20 mM
4	sodium chloride	natural abundance	50 mM
5	TERBIUM(III) ION	natural abundance	0.5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.5 mM
7	entity_2	[U-99% 13C; U-99% 15N]	0.5 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	TERBIUM(III) ION	natural abundance	0.5 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2KTR, Strand ID: A, B Detail

Release date

2010-05-02

Citation

PCS-based structure determination of protein-protein complexes
Saio, T., Yokochi, M., Kumeta, H., Inagaki, F.
J. Biomol. NMR (2010), 46, 271-280, PubMed 20300805 , DOI 10.1007/s10858-010-9401-4 , Abstract

Related entities 1. P62 PB1 Dimer, entity 1, : 2 : 10 entities Detail

Related entities 2. P62 PB1 Dimer, entity 2, : 2 : 21 entities Detail

Interaction partners 1. P62 PB1 Dimer, entity 1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Interaction partners 2. P62 PB1 Dimer, entity 2, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC