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BMRB: 16749

2L73

Backbone Assignments of the PX Domain of Noxo1b

Authors

Davis, N.Y., Horita, D.A., McPhail, L.C.

Assembly

Noxo1b PX domain

Entity

1. Noxo1b PX domain (polymer, Thiol state: not present), 149 monomers, 16579.98 Da Detail

GPLGSMAGPR YPVSVQGAAL VQIKRLQTFA FSVRWSDGSD TFVRRSWDEF RQLKKTLKET FPVEAGLLRR SDRVLPKLLD APLLGRVGRT SRGLARLQLL ETYSRRLLAT AERVARSPTI TGFFAPQPLD LEPALPPGSR VILPTPEEQ

Formula weight

16579.98 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 42.0 %, Completeness (bb): 78.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.0 % (738 of 1756)	20.6 % (190 of 922)	60.9 % (420 of 690)	88.9 % (128 of 144)
Backbone	78.3 % (678 of 866)	48.8 % (144 of 295)	93.1 % (406 of 436)	94.8 % (128 of 135)
Sidechain	17.9 % (184 of 1028)	7.3 % (46 of 627)	35.2 % (138 of 392)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 110)	0.0 % (0 of 55)	0.0 % (0 of 53)	0.0 % (0 of 2)
Methyl	8.7 % (16 of 184)	5.4 % (5 of 92)	12.0 % (11 of 92)

1. Noxo1b PX

GPLGSMAGPR YPVSVQGAAL VQIKRLQTFA FSVRWSDGSD TFVRRSWDEF RQLKKTLKET FPVEAGLLRR SDRVLPKLLD APLLGRVGRT SRGLARLQLL ETYSRRLLAT AERVARSPTI TGFFAPQPLD LEPALPPGSR VILPTPEEQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	Noxo1b PX	[U-13C; U-15N; U-2H]	500 uM
2	sodium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	90 %
6	glycerol	natural abundance	5 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.82 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.82 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 2 Detail

Release date

2011-03-07

Citation

Backbone 1H, 15N, and 13C resonance assignments for the NOXO1β PX domain
Davis, N.Y., McPhail, L.C., Horita, D.A.
Biomol. NMR Assign. (2011), 5, 139-141, PubMed 21188560 , DOI 10.1007/s12104-010-9286-x , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17343 released on 2011-03-29
Title Structure of the NOXO1b PX domain

Related entities 1. Noxo1b PX domain, : 1 : 15 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC