BMRB: 16761

Solution structue of the N-domain of Wilson disease protein: Distinct nucleotide-binding environment and effects of disease mutations

Authors

Dmtriev, O., Tsivkovski, R., Abildgaard, F., Morgan, C.T., Markley, J.L., Lutsenko, S.

Assembly

ATP7B N-domain/ATP complex

Entity

1. ATP7B (polymer, Thiol state: not reported), 165 monomers, 17291.75 Da Detail

AGHMVPRVMR VLLLGDVATL PLRKVLAVVG TAEASSEHPL GVAVTKYCKE ELGTETLGYC TDFQAVPGCG IGCKVSNVEG ILAHSERPLS APASHLNEAG SLPAEKDAVP QTFSVLIGNR EWLRRNGLTI SSDVSDAMTD HEMKGQTAIL VAIDGVLCGM IAIAD

2. ATP (non-polymer), 507.181 Da

Total weight

17798.932 Da

Max. entity weight

17291.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

binding_constants

Protein Blocks Logo

Calculated from 10 models in PDB: 2ARF, Strand ID: A Detail

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Binding constant

1 Kd values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 6.0 Detail

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List #1 binding_data, Participants 1. ATP7B, 2. ATP,

Sample ID	Exp. name	Binding reaction	Obs. entity ID	Obs. parameter	Value type	Value	Value error
2	2D 1H-15N HSQC	1 + 2 ⇄ 12	1	chemical shifts	Kd	70 uM

Release date

2010-03-23

Citation

Solution structure of the N-domain of Wilson disease protein: distinct nucleotide-binding environment and effects of disease mutations
Dmtriev, O., Tsivkovski, R., Abildgaard, F., Morgan, C.T., Markley, J.L., Lutsenko, S.
Proc. Natl. Acad. Sci. U. S. A. (2006), 103, 5302-5307, PubMed 16567646 , DOI 10.1073/pnas.0507416103 , Abstract

Related entities 1. ATP7B, : 1 : 2 : 85 entities Detail

Interaction partners 1. ATP7B, : 2 interactors Detail

Experiments performed 2 experiments Detail