BMRBj - BMREntryMaster

	Total	¹H
All	75.9 % (154 of 203)	75.9 % (154 of 203)
Backbone	93.9 % (62 of 66)	93.9 % (62 of 66)
Sidechain	67.2 % (92 of 137)	67.2 % (92 of 137)
Aromatic	40.0 % (2 of 5)	40.0 % (2 of 5)
Methyl	76.0 % (19 of 25)	76.0 % (19 of 25)

1. Fst

MKDLMSLVIA PIFVGLVLEM ISRVLDEEDD SRK

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.0, Details 10 mM DCP (dodecylphosphocholine

#	Name	Isotope labeling	Concentration
1	Fst_toxin	natural abundance	.5 mM
2	potassium phosphate	natural abundance	50 mM
3	DPC	[U-99% 2H]	100 mM
4	sodium azide	natural abundance	0.02 %
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

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Calculated from 20 models in PDB: 2KV5, Strand ID: A Detail

Release date

2010-08-11

Citation

Solution structure and membrane binding of the toxin fst of the par addiction module
Gobl, C., Kosol, S., Stockner, T., Ruckert, H.M., Zangger, K.
Biochemistry (2010), 49, 6567-6575, PubMed 20677831 , DOI 10.1021/bi1005128 , Abstract

Related entities 1. par toxin Fst in DPC micelles, : 1 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	193		97.0 (chain: 1, length: 33)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
33	LYS	HZ1	7.4893
33	LYS	HZ2	7.4893
33	LYS	HZ3	7.4893

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	203	152	74.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	66	63	95.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	137	89	65.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	28	19	67.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	5	2	40.0

Keywords bacterial, plasmid, toxin-antitoxin

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16769_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (0.352 seconds)

BMRB: 16769

Sample

Related entities 1. par toxin Fst in DPC micelles, : 1 entities Detail

Experiments performed 2 experiments Detail

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Sample

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Sample

Chemical shift validation 3 contents Detail