Solution structure of the CBX7 chromodomain in complex with a H3K27me2 peptide
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.2 % (915 of 1062) | 94.0 % (533 of 567) | 76.1 % (312 of 410) | 82.4 % (70 of 85) |
Backbone | 80.4 % (405 of 504) | 97.6 % (166 of 170) | 68.1 % (173 of 254) | 82.5 % (66 of 80) |
Sidechain | 92.3 % (591 of 640) | 92.4 % (367 of 397) | 92.4 % (220 of 238) | 80.0 % (4 of 5) |
Aromatic | 89.0 % (73 of 82) | 100.0 % (41 of 41) | 76.3 % (29 of 38) | 100.0 % (3 of 3) |
Methyl | 100.0 % (78 of 78) | 100.0 % (39 of 39) | 100.0 % (39 of 39) |
1. CBX7 chromodomain
MELSAIGEQV FAVESIRKKR VRKGKVEYLV KWKGWPPKYS TWEPEEHILD PRLVMAYEEK EERDRASGYR K2. H3K27me2 peptide
APRKQLATKA ARXSAPSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CBX7 chromodomain | [U-100% 15N] | 0.5 mM | |
2 | H3K27me2 peptide | natural abundance | 0.75 mM | |
3 | sodium phosphate | natural abundance | 4.3 mM | |
4 | potassium phosphate | natural abundance | 1.4 mM | |
5 | DTT | natural abundance | 5 mM | |
6 | sodium chloride | natural abundance | 137 mM | |
7 | potassium chloride | natural abundance | 3 mM | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | CBX7 chromodomain | [U-100% 13C; U-100% 15N] | 0.5 mM | |
11 | H3K27me2 peptide | natural abundance | 0.75 mM | |
12 | sodium phosphate | natural abundance | 4.3 mM | |
13 | potassium phosphate | natural abundance | 1.4 mM | |
14 | DTT | natural abundance | 5 mM | |
15 | sodium chloride | natural abundance | 137 mM | |
16 | potassium chloride | natural abundance | 3 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | H3K27me2 peptide | natural abundance | 0.5 mM | |
20 | sodium phosphate | natural abundance | 4.3 mM | |
21 | potassium phosphate | natural abundance | 1.4 mM | |
22 | DTT | natural abundance | 5 mM | |
23 | sodium chloride | natural abundance | 137 mM | |
24 | potassium chloride | natural abundance | 3 mM | |
25 | H2O | natural abundance | 90 % | |
26 | D2O | natural abundance | 10 % |