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Found 1 Document (3.726 seconds)

BMRB: 16796

2LE8

Solution NMR structure of the Cdt1 binding domain(CBD) in complex with the MCM6 binding domain (MBD)

Authors

LIU, C., WEI, Z., ZHU, G.

Assembly

Cdt1 binding domain(CBD) in complex with the MCM6 binding domain (MBD)

Entity

1. CBD (polymer, Thiol state: not present), 114 monomers, 13285.73 Da Detail

APKASLRLGF SEYSRISNLI VLHLRKVEEE EDESALKRSE LVNWYLKEIE SEIDSEEELI NKKRIIEKVI HRLTHYDHVL IELTQAGLKG STEGSESYEE DPYLVVNPNY LLED

2. MBD (polymer, Thiol state: not present), 30 monomers, 3353.847 Da Detail

SPSALKGVSQ DLLERIRAKE AQKQLAQMTR

Total weight

16639.578 Da

Max. entity weight

13285.73 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 67.2 %, Completeness (bb): 75.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.2 % (1157 of 1722)	71.6 % (647 of 904)	57.3 % (382 of 667)	84.8 % (128 of 151)
Backbone	75.4 % (645 of 856)	89.6 % (259 of 289)	61.6 % (263 of 427)	87.9 % (123 of 140)
Sidechain	62.9 % (632 of 1005)	63.1 % (388 of 615)	63.1 % (239 of 379)	45.5 % (5 of 11)
Aromatic	25.6 % (22 of 86)	25.6 % (11 of 43)	23.8 % (10 of 42)	100.0 % (1 of 1)
Methyl	76.7 % (135 of 176)	77.3 % (68 of 88)	76.1 % (67 of 88)

1. CBD

APKASLRLGF SEYSRISNLI VLHLRKVEEE EDESALKRSE LVNWYLKEIE SEIDSEEELI NKKRIIEKVI HRLTHYDHVL IELTQAGLKG STEGSESYEE DPYLVVNPNY LLED

2. MBD

SPSALKGVSQ DLLERIRAKE AQKQLAQMTR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	CBD	[U-100% 15N]		0.6 mM
2	MBD	[U-100% 15N]		1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	CBD	[U-100% 13C]		0.6 mM
4	MBD	[U-100% 13C]		1 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
8	MBD	natural abundance		1.0 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
9	CBD	natural abundance		1.0 mM
10	MBD	[U-100% 13C; U-100% 15N]		0.8 mM

Release date

2011-05-18

Citation

Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6
Wei, Z., Liu, C., Wu, X., Xu, N., Zhou, B., Liang, C., Zhu, G.
J. Biol. Chem. (2010), 285, 12469-12473, PubMed 20202939 , DOI 10.1074/jbc.C109.094599 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 16396 released on 2010-05-19
    Title The solution structure of CBD of human MCM6
  Swiss-Prot: Q14566 released on 1997-11-01
    Title MCM6_HUMAN Entity DNA replication licensing factor MCM6

Related entities 1. CBD, : 1 : 1 : 2 : 24 entities Detail

Related entities 2. MBD, : 1 : 3 : 8 entities Detail

Interaction partners 1. CBD, : 69 : 1 interactors Detail

More details for 70 interactors identified by 26 citations

Interaction partners 2. MBD, : 16 : 1 interactors Detail

More details for 17 interactors identified by 8 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	CBD	[U-100% 15N]		0.6 mM
2	MBD	[U-100% 15N]		1 mM

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	CBD	[U-100% 13C]		0.6 mM
4	MBD	[U-100% 13C]		1 mM

3 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

4 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

5 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

6 3D HBHA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

7 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

8 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
6	MBD	[U-100% 13C; U-100% 15N]		1 mM

9 3D HCCH-COSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	CBD	[U-100% 13C]		0.6 mM
4	MBD	[U-100% 13C]		1 mM

10 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	CBD	[U-100% 13C]		0.6 mM
4	MBD	[U-100% 13C]		1 mM

11 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	CBD	[U-100% 15N]		0.6 mM
2	MBD	[U-100% 15N]		1 mM

12 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	CBD	[U-100% 13C]		0.6 mM
4	MBD	[U-100% 13C]		1 mM

13 13C-edited (F1 ), 13C/15N-filtered (F3)3D NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	CBD	[U-100% 13C; U-100% 15N]		0.6 mM
8	MBD	natural abundance		1.0 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16796_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16796_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1264		99.1 (chain: 1, length: 114), 100.0 (chain: 2, length: 30)
1	Chemical shift references	chemical_shift_reference_1	OK	4		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 114, Sequence coverage: 99.1%
  - Entity_assembly_ID: 2, Chain length: 30, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 774
  - ¹³C chemical shifts: 364
  - ¹⁵N chemical shifts: 126
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	714	490	68.6
¹³C chemical shifts	535	282	52.7
¹⁵N chemical shifts	124	104	83.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	229	216	94.3
¹³C chemical shifts	228	109	47.8
¹⁵N chemical shifts	111	103	92.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	485	274	56.5
¹³C chemical shifts	307	173	56.4
¹⁵N chemical shifts	13	1	7.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	48	67.6
¹³C chemical shifts	71	47	66.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	11	25.6
¹³C chemical shifts	42	10	23.8
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	190	152	80.0
¹³C chemical shifts	132	82	62.1
¹⁵N chemical shifts	36	22	61.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	60	46	76.7
¹³C chemical shifts	60	23	38.3
¹⁵N chemical shifts	29	19	65.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	130	106	81.5
¹³C chemical shifts	72	59	81.9
¹⁵N chemical shifts	7	3	42.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	18	100.0
¹³C chemical shifts	18	18	100.0

Keywords Cdt1, Mcm6, NMR

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Found 1 Document (3.726 seconds)

BMRB: 16796

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. CBD, : 1 : 1 : 2 : 24 entities Detail

Related entities 2. MBD, : 1 : 3 : 8 entities Detail

Interaction partners 1. CBD, : 69 : 1 interactors Detail

Interaction partners 2. MBD, : 16 : 1 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 4 contents Detail