BMRBj - BMREntryMaster

Found 1 Document (1.092 seconds)

BMRB: 16806

2N47

Solution NMR Structure of the Slr1183 protein from Synechocystis sp. PCC 6803. Northeast Structural Genomics Consortium Target Target SgR145.

Authors

Xiao, R., Rossi, P., Forouhar, F.L., Lange, O., Lee, H., Lemak, A.L., Belote, R., Ciccosanti, C.L., Foote, E.L., Sahdev, S., Acton, T., Everett, J., Montelione, G.

Assembly

SgR145

Entity

1. SgR145 (polymer, Thiol state: all free), 202 monomers, 22380.04 Da Detail

MWDERFSQSE YVYGTEPNDF LVSVANQIPQ GKILCLAEGE GRNACFLASL GYEVTAVDQS SVGLAKAKQL AQEKGVKITT VQSNLADFDI VADAWEGIVS IFCHLPSSLR QQLYPKVYQG LKPGGVFILE GFAPEQLQYN TGGPKDLDLL PKLETLQSEL PSLNWLIANN LERNLDEGAY HQGKAALIQL LGQKLEHHHH HH

Formula weight

22380.04 Da

Source organism

Synechocystis sp. PCC 6803

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 87.1 %, Completeness: 47.7 %, Completeness (bb): 70.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.7 % (1125 of 2360)	29.4 % (359 of 1223)	65.6 % (601 of 916)	74.7 % (165 of 221)
Backbone	70.9 % (847 of 1194)	46.6 % (192 of 412)	83.7 % (493 of 589)	83.9 % (162 of 193)
Sidechain	32.1 % (434 of 1351)	20.6 % (167 of 811)	51.6 % (264 of 512)	10.7 % (3 of 28)
Aromatic	4.6 % (9 of 194)	5.2 % (5 of 97)	1.1 % (1 of 94)	100.0 % (3 of 3)
Methyl	80.0 % (192 of 240)	73.3 % (88 of 120)	86.7 % (104 of 120)

1. SgR145

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KW5, Strand ID: A Detail

Release date

2010-04-27

Related entities 1. SgR145, : 1 : 2 : 4 entities Detail

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

3 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

5 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

6 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

7 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

8 3D HNCA(CO)

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

9 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

12 3D 1H-13C HSQC_NOESY_HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

13 3D 1H-15N HSQC_NOESY_HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

14 3D 1H-13C-15N HSQC_NOESY_HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

15 3D 1H-15N-13C HSQC_NOESY_HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

16 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

17 1D 15N T1

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

18 1D 15N T2 CPMG

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

19 2D- HET NOE

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SgR145	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

nullKeywords NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-2, Protein NMR, Protein Structure Initiative, Structural Genomics, Target SgR145

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Found 1 Document (1.092 seconds)

BMRB: 16806

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SgR145, : 1 : 2 : 4 entities Detail

Experiments performed 19 experiments Detail

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