BMRBj - BMREntryMaster

Found 1 Document (0.794 seconds)

BMRB: 16814

2KVF

Structure of the three-Cys2His2 domain of mouse testis zinc finger protein

Authors

Chou, C., Lou, Y., Chen, C.

Assembly

mTZD finger1

Entity

1. mTZD finger1 (polymer, Thiol state: all other bound), 28 monomers, 3339.851 Da Detail

MRPYSCSVCG KRFSLKHQME THYRVHTG

2. ZN (non-polymer, Thiol state: not present), 65.409 Da

Total weight

3405.26 Da

Max. entity weight

3339.851 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 86.2 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (288 of 334)	86.4 % (152 of 176)	84.6 % (110 of 130)	92.9 % (26 of 28)
Backbone	91.6 % (152 of 166)	93.0 % (53 of 57)	90.2 % (74 of 82)	92.6 % (25 of 27)
Sidechain	82.5 % (160 of 194)	83.2 % (99 of 119)	81.1 % (60 of 74)	100.0 % (1 of 1)
Aromatic	68.4 % (26 of 38)	73.7 % (14 of 19)	63.2 % (12 of 19)
Methyl	100.0 % (16 of 16)	100.0 % (8 of 8)	100.0 % (8 of 8)

1. mTZD finger1

MRPYSCSVCG KRFSLKHQME THYRVHTG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Release date

2010-07-26

Citation

Structure and DNA binding characteristics of the three-Cys(2)His(2) domain of mouse testis zinc finger protein
Chou, C., Lou, Y., Tang, T.K., Chen, C.
Proteins (2010), 78, 2202-2212, PubMed 20544958 , DOI 10.1002/prot.22732 , Abstract

Related entities 1. mTZD finger1, : 1 : 3 : 1829 entities Detail

Interaction partners 1. mTZD finger1, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

5 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

7 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

11 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16814_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16814_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assignments_zf1	Warning	304		92.9 (chain: 1, length: 28)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assignments_zf1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 28, Sequence coverage: 92.9%
- Total number of assignments
  - ¹H chemical shifts: 168
  - ¹³C chemical shifts: 110
  - ¹⁵N chemical shifts: 26
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords DNA, protein