BMRBj - BMREntryMaster

Found 1 Document (0.624 seconds)

BMRB: 16815

2KVG

Structure of the three-Cys2His2 domain of mouse testis zinc finger protein

Authors

Chou, C., Lou, Y., Chen, C.

Assembly

mTZD finger3

Entity

1. mTZD finger3 (polymer, Thiol state: free and other bound), 27 monomers, 2872.357 Da Detail

AAPYRCPLCR AGCPSLASMQ AHMRGHS

2. ZN (non-polymer, Thiol state: not present), 65.409 Da

Total weight

2937.7659 Da

Max. entity weight

2872.357 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.0 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.0 % (267 of 284)	93.3 % (139 of 149)	93.6 % (103 of 110)	100.0 % (25 of 25)
Backbone	100.0 % (156 of 156)	100.0 % (53 of 53)	100.0 % (79 of 79)	100.0 % (24 of 24)
Sidechain	88.9 % (136 of 153)	89.6 % (86 of 96)	87.5 % (49 of 56)	100.0 % (1 of 1)
Aromatic	68.8 % (11 of 16)	87.5 % (7 of 8)	50.0 % (4 of 8)
Methyl	100.0 % (18 of 18)	100.0 % (9 of 9)	100.0 % (9 of 9)

1. mTZD finger3

AAPYRCPLCR AGCPSLASMQ AHMRGHS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Release date

2010-07-26

Citation

Structure and DNA binding characteristics of the three-Cys(2)His(2) domain of mouse testis zinc finger protein
Chou, C., Lou, Y., Tang, T.K., Chen, C.
Proteins (2010), 78, 2202-2212, PubMed 20544958 , DOI 10.1002/prot.22732 , Abstract

Related entities 1. mTZD finger3, : 1 : 3 : 4 : 251 entities Detail

Interaction partners 1. mTZD finger3, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

4 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

5 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

6 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	[U-99% 2H]		50 mM
2	sodium chloride	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16815_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16815_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assignments_zf3	Warning	285		100.0 (chain: 1, length: 27)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assignments_zf3
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 27, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 157
  - ¹³C chemical shifts: 103
  - ¹⁵N chemical shifts: 25
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords protein/DNA