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BMRB: 16833

2LOY

Minimal Constraint Solution NMR Structure of Translationally-controlled tumor protein (TCTP) from C.elegans, Northeast Structural Genomics Consortium Target Target WR73

Authors

Aramini, J.M., Rossi, P., Cort, J.R., Cooper, B., Maglaqui, M., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T.

Assembly

WR73

Entity

1. WR73 (polymer, Thiol state: all free), 183 monomers, 20784.42 Da Detail

MLIYKDIFTD DELSSDSFPM KLVDDLVYEF KGKHVVRKEG EIVLAGSNPS AEEGAEDDGS DEHVERGIDI VLNHKLVEMN CYEDASMFKA YIKKFMKNVI DHMEKNNRDK ADVDAFKKKI QGWVVSLLAK DRFKNLAFFI GERAAEGAEN GQVAIIEYRD VDGTEVPTLM LVKEAIIEEK CLE

Formula weight

20784.42 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 58.0 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	58.0 % (1249 of 2152)	39.7 % (445 of 1120)	73.2 % (616 of 841)	98.4 % (188 of 191)
Backbone	83.7 % (914 of 1092)	55.3 % (207 of 374)	98.5 % (530 of 538)	98.3 % (177 of 180)
Sidechain	40.9 % (504 of 1232)	31.8 % (237 of 746)	53.9 % (256 of 475)	100.0 % (11 of 11)
Aromatic	38.0 % (60 of 158)	68.4 % (54 of 79)	6.4 % (5 of 78)	100.0 % (1 of 1)
Methyl	76.2 % (154 of 202)	70.3 % (71 of 101)	82.2 % (83 of 101)

1. WR73

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.82 mM [U-13C; U-15N; U-2H; ILVFY-1H] WR73, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	WR73	[U-13C; U-15N; U-2H; ILVFY-1H]	0.82 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM
7	sodium azide	natural abundance	0.02 %
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.85 mM [U-5%-13C; U-100%-15N] WR73, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	WR73	[U-5% 13C; U-100% 15N]	0.85 mM
11	MES	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	calcium chloride	natural abundance	5 mM
14	DTT	natural abundance	10 mM
15	DSS	natural abundance	50 uM
16	sodium azide	natural abundance	0.02 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LOY, Strand ID: A Detail

Release date

2010-04-28

Related entities 1. WR73, : 1 : 1 : 2 : 109 entities Detail

Interaction partners 1. WR73, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC