Solution structure of a novel Ubiquitin-binding domain from Human PLAA (PFUC, Gly76-Pro77 cis isomer).
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 74.5 % (711 of 954) | 79.9 % (397 of 497) | 61.4 % (226 of 368) | 98.9 % (88 of 89) |
Backbone | 82.1 % (386 of 470) | 96.9 % (157 of 162) | 66.1 % (154 of 233) | 100.0 % (75 of 75) |
Sidechain | 70.7 % (394 of 557) | 71.6 % (240 of 335) | 67.8 % (141 of 208) | 92.9 % (13 of 14) |
Aromatic | 15.7 % (16 of 102) | 29.4 % (15 of 51) | 0.0 % (0 of 50) | 100.0 % (1 of 1) |
Methyl | 83.8 % (62 of 74) | 86.5 % (32 of 37) | 81.1 % (30 of 37) |
1. PFUC-cis
ANQQTSGKVL YEGKEFDYVF SIDVNEGGPS YKLPYNTSDD PWLTAYNFLQ KNDLNPMFLD QVAKFIIDNT KGQMLGLGNPSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PFUC | natural abundance | 1 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |