BMRBj - BMREntryMaster

Found 1 Document (1.488 seconds)

BMRB: 16879

6GBE

Chemical shift assignments of the 3rd PDZ domain of protein tyrosine phosphatase basophil like (PTP-BL)

Authors

Kock, G., Dicks, M., Stoll, R.

Assembly

PDZ3 PTP-BL

Entity

1. PDZ3 PTP-BL (polymer, Thiol state: not present), 119 monomers, 12677.24 Da Detail

TPHVKDYSFV TEDNTFEVKL FKNSSGLGFS FSREDNLIPE QINGSIVRVK KLFPGQPAAE SGKIDVGDVI LKVNGAPLKG LSQQDVISAL RGTAPEVSLL LCRPAPGVLP EIDTPGNSS

Formula weight

12677.24 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 75.6 %, Completeness (bb): 89.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.6 % (1022 of 1351)	75.2 % (529 of 703)	73.7 % (390 of 529)	86.6 % (103 of 119)
Backbone	89.5 % (621 of 694)	92.1 % (220 of 239)	87.3 % (302 of 346)	90.8 % (99 of 109)
Sidechain	65.2 % (499 of 765)	66.6 % (309 of 464)	63.9 % (186 of 291)	40.0 % (4 of 10)
Aromatic	16.7 % (12 of 72)	33.3 % (12 of 36)	0.0 % (0 of 36)
Methyl	78.2 % (111 of 142)	81.7 % (58 of 71)	74.6 % (53 of 71)

1. PDZ3 PTP-BL

TPHVKDYSFV TEDNTFEVKL FKNSSGLGFS FSREDNLIPE QINGSIVRVK KLFPGQPAAE SGKIDVGDVI LKVNGAPLKG LSQQDVISAL RGTAPEVSLL LCRPAPGVLP EIDTPGNSS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PDZ3	[U-15N]	0.5 ~ 1.0 mM
2	TSP	natural abundance	1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	potassium phosphate	natural abundance	1.4 mM
6	potassium chloride	natural abundance	2.7 mM
7	sodium chloride	natural abundance	137 mM
8	sodium phosphate	natural abundance	9 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	PDZ3	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
10	TSP	natural abundance	1.0 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %
13	potassium phosphate	natural abundance	1.4 mM
14	potassium chloride	natural abundance	2.7 mM
15	sodium chloride	natural abundance	137 mM
16	sodium phosphate	natural abundance	9 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6GBD, Strand ID: A Detail

Release date

2010-07-26

Citation

Sequence-specific 1H, 13C, and 15N assignment of the extended PDZ3 domain of the protein tyrosine phosphatase basophil-like PTP-BL
Kock, G., Dicks, M., Heumann, R., Erdmann, K.S., Stoll, R.
Biomol. NMR Assign. (2010), 4, 199-202, PubMed 20563762 , DOI 10.1007/s12104-010-9242-9 , Abstract

Related entities 1. PDZ3 PTP-BL, : 1 : 1 : 2 : 145 entities Detail

Interaction partners 1. PDZ3 PTP-BL, : 6 interactors Detail

More details for 6 interactors identified by 5 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC