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BMRB: 16883

4FA0

the N-terminal domain of human H-REV107

Authors

Ren, X., Xia, B.

Assembly

H-REV107N

Entity

1. H-REV107N (polymer, Thiol state: all free), 125 monomers, 13948.77 Da Detail

MRAPIPEPKP GDLIEIFRPF YRHWAIYVGD GYVVHLAPPS EVAGAGAASV MSALTDKAIV KKELLYDVAG SDKYQVNNKH DDKYSPLPCS KIIQRAEELV GQEVLYKLTS ENCEHFVNEL RYGVA

Formula weight

13948.77 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 88.9 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.9 % (1295 of 1456)	86.6 % (655 of 756)	90.6 % (522 of 576)	95.2 % (118 of 124)
Backbone	94.1 % (689 of 732)	94.0 % (234 of 249)	94.0 % (345 of 367)	94.8 % (110 of 116)
Sidechain	85.6 % (720 of 841)	83.0 % (421 of 507)	89.3 % (291 of 326)	100.0 % (8 of 8)
Aromatic	55.7 % (68 of 122)	55.7 % (34 of 61)	55.0 % (33 of 60)	100.0 % (1 of 1)
Methyl	95.8 % (138 of 144)	94.4 % (68 of 72)	97.2 % (70 of 72)

1. H-REV107N

MRAPIPEPKP GDLIEIFRPF YRHWAIYVGD GYVVHLAPPS EVAGAGAASV MSALTDKAIV KKELLYDVAG SDKYQVNNKH DDKYSPLPCS KIIQRAEELV GQEVLYKLTS ENCEHFVNEL RYGVA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	H-REV107N	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	30 mM
3	sodium chloride	natural abundance	30 mM
4	DTT	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %
7	sodium azide	natural abundance	0.05 w/v
8	DSS	natural abundance	0.02 w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Release date

2010-06-14

Citation

1H, 13C and 15N resonance assignments of human H-REV107 N-terminal domain
Ren, X., Lin, J., Jin, C., Xia, B.
Biomol. NMR Assign. (2010), 4, 175-178, PubMed 20526701 , DOI 10.1007/s12104-010-9238-5 , Abstract

Related entities 1. H-REV107N, : 1 : 6 : 1 : 57 entities Detail

Interaction partners 1. H-REV107N, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC