BMRBj - BMREntryMaster

Found 1 Document (0.996 seconds)

BMRB: 16888

2K4H

Solution structure of the HIV-2 myristoylated Matrix protein

Authors

Saad, J.S., Ablan, S.D., Ghanam, R.H., Kim, A., Andrews, K., Nagashima, K., Freed, E.O., Summers, M.F.

Assembly

HIV-2 myristoylated matrix protein

Entity

1. HIV-2 myristoylated matrix protein (polymer, Thiol state: all free), 135 monomers, 15081.38 Da Detail

XGARNSVLRG KKADELERIR LRPGGKKKYR LKHIVWAANK LDRFGLAESL LESKEGCQKI LTVLDPMVPT GSENLKSLFN TVCVIWCIHA EEKVKDTEGA KQIVRRHLVA ETGTAEKMPS TSRPTAPSSE KGGNY

Formula weight

15081.38 Da

Source organism

Human immunodeficiency virus 2

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 71.7 %, Completeness (bb): 75.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.7 % (1120 of 1563)	73.6 % (606 of 823)	64.0 % (386 of 603)	93.4 % (128 of 137)
Backbone	75.0 % (594 of 792)	90.5 % (247 of 273)	57.3 % (224 of 391)	96.1 % (123 of 128)
Sidechain	70.0 % (626 of 894)	65.3 % (359 of 550)	78.2 % (262 of 335)	55.6 % (5 of 9)
Aromatic	52.8 % (38 of 72)	52.8 % (19 of 36)	50.0 % (17 of 34)	100.0 % (2 of 2)
Methyl	93.9 % (139 of 148)	94.6 % (70 of 74)	93.2 % (69 of 74)

1. HIV-2 myristoylated matrix protein

XGARNSVLRG KKADELERIR LRPGGKKKYR LKHIVWAANK LDRFGLAESL LESKEGCQKI LTVLDPMVPT GSENLKSLFN TVCVIWCIHA EEKVKDTEGA KQIVRRHLVA ETGTAEKMPS TSRPTAPSSE KGGNY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
9	HIV-2 myristoylated matrix protein	natural abundance	0.8 mM
10	potassium phosphate	natural abundance	20 mM
11	DTT	natural abundance	10 mM
12	Glutamate	natural abundance	50 mM
13	Aspartate	natural abundance	50 mM
14	sodium chloride	natural abundance	100 mM
15	H2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	HIV-2 myristoylated matrix protein	[U-13C; U-15N]	0.8 mM
17	potassium phosphate	natural abundance	20 mM
18	DTT	natural abundance	10 mM
19	Glutamate	natural abundance	50 mM
20	Aspartate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.706 ppm	external	direct	1.0
¹⁵N	urea	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 2.55 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.706 ppm	external	direct	1.0
¹⁵N	urea	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 2.55 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbon	0.0 ppm	external	direct	1.0
¹H	water	protons	4.706 ppm	external	direct	1.0
¹⁵N	urea	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2K4H, Strand ID: A Detail

Release date

2012-08-01

Citation

Structure of the myristylated human immunodeficiency virus type 2 matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in membrane targeting
Saad, J.S., Ablan, S.D., Ghanam, R.H., Kim, A., Andrews, K., Nagashima, K., Soheilian, F., Freed, E.O., Summers, M.F.
J. Mol. Biol. (2008), 382, 434-447, PubMed 18657545 , DOI 10.1016/j.jmb.2008.07.027 , Abstract

Related entities 1. HIV-2 myristoylated matrix protein, : 1 : 4 : 83 entities Detail

Interaction partners 1. HIV-2 myristoylated matrix protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

3 2D 1H-1H NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
9	HIV-2 myristoylated matrix protein	natural abundance	0.8 mM
10	potassium phosphate	natural abundance	20 mM
11	DTT	natural abundance	10 mM
12	Glutamate	natural abundance	50 mM
13	Aspartate	natural abundance	50 mM
14	sodium chloride	natural abundance	100 mM
15	H2O	natural abundance	100 %

4 3D HNCA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	HIV-2 myristoylated matrix protein	[U-13C; U-15N]	0.8 mM
17	potassium phosphate	natural abundance	20 mM
18	DTT	natural abundance	10 mM
19	Glutamate	natural abundance	50 mM
20	Aspartate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

5 3D HN(CO)CA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	HIV-2 myristoylated matrix protein	[U-13C; U-15N]	0.8 mM
17	potassium phosphate	natural abundance	20 mM
18	DTT	natural abundance	10 mM
19	Glutamate	natural abundance	50 mM
20	Aspartate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

6 3D 1H-15N TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

nullKeywords Protein

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Found 1 Document (0.996 seconds)

BMRB: 16888

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HIV-2 myristoylated matrix protein, : 1 : 4 : 83 entities Detail

Interaction partners 1. HIV-2 myristoylated matrix protein, : 1 interactors Detail

Experiments performed 6 experiments Detail

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