BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	97.5 % (987 of 1012)	98.3 % (528 of 537)	96.1 % (365 of 380)	98.9 % (94 of 95)
Backbone	97.1 % (468 of 482)	98.2 % (160 of 163)	95.8 % (230 of 240)	98.7 % (78 of 79)
Sidechain	98.2 % (597 of 608)	98.4 % (368 of 374)	97.7 % (213 of 218)	100.0 % (16 of 16)
Aromatic	87.5 % (70 of 80)	87.5 % (35 of 40)	86.1 % (31 of 36)	100.0 % (4 of 4)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. VARC244

GPLGSMNKFT DDEWNQLKQD FISGILENEQ KDLVAKLTNS DPIMNQLDLL HKWLDRHRDM CEKWKSKEDI LHKLNEQWNK D

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	VARC244	[U-100% 15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-2H]	5 %
4	DTT	natural abundance	1 mM
5	DSS	natural abundance	0.1 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	sodium azide	natural abundance	0.01 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	VARC244	[U-13C; U-15N]	1 mM
18	D2O	[U-2H]	100 %
19	DTT	natural abundance	1 mM
20	DSS	natural abundance	0.1 mM
21	sodium phosphate	natural abundance	20 mM
22	sodium chloride	natural abundance	50 mM
23	sodium azide	natural abundance	0.01 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 39 models in PDB: 2LKL, Strand ID: A Detail

Release date

2012-01-17

Citation

Structural analysis of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) intracellular domain reveals a conserved interaction epitope
Mayer, C., Slater, L., Erat, M.C., Konrat, R., Vakonakis, I.
J. Biol. Chem. (2012), 287, 7182-7189, PubMed 22249178 , DOI 10.1074/jbc.M111.330779 , Abstract

Related entities 1. VARC244, : 1 : 11 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	VARC244	[U-100% 15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-2H]	5 %
4	DTT	natural abundance	1 mM
5	DSS	natural abundance	0.1 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	sodium azide	natural abundance	0.01 %

2 2D 1H-13C HSQC

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	VARC244	[U-13C; U-15N]	1 mM
18	D2O	[U-2H]	100 %
19	DTT	natural abundance	1 mM
20	DSS	natural abundance	0.1 mM
21	sodium phosphate	natural abundance	20 mM
22	sodium chloride	natural abundance	50 mM
23	sodium azide	natural abundance	0.01 %

3 3D CBCA(CO)NH

Instrument

Home-built OMEGA - 600 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

4 3D C(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

5 3D HNCO

Instrument

Home-built OMEGA - 600 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

6 3D HNCACB

Instrument

Home-built OMEGA - 600 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

7 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

8 3D HCCH-COSY

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	VARC244	[U-13C; U-15N]	1 mM
18	D2O	[U-2H]	100 %
19	DTT	natural abundance	1 mM
20	DSS	natural abundance	0.1 mM
21	sodium phosphate	natural abundance	20 mM
22	sodium chloride	natural abundance	50 mM
23	sodium azide	natural abundance	0.01 %

9 3D HNHB

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	VARC244	[U-100% 15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-2H]	5 %
4	DTT	natural abundance	1 mM
5	DSS	natural abundance	0.1 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	sodium azide	natural abundance	0.01 %

10 3D HNHA

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	VARC244	[U-100% 15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-2H]	5 %
4	DTT	natural abundance	1 mM
5	DSS	natural abundance	0.1 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	sodium azide	natural abundance	0.01 %

11 3D 1H-13C NOESY

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	VARC244	[U-13C; U-15N]	1 mM
18	D2O	[U-2H]	100 %
19	DTT	natural abundance	1 mM
20	DSS	natural abundance	0.1 mM
21	sodium phosphate	natural abundance	20 mM
22	sodium chloride	natural abundance	50 mM
23	sodium azide	natural abundance	0.01 %

12 3D 1H-15N NOESY

Instrument

Home-built OMEGA - 950 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	VARC244	[U-100% 15N]	1 mM
2	H2O	natural abundance	95 %
3	D2O	[U-2H]	5 %
4	DTT	natural abundance	1 mM
5	DSS	natural abundance	0.1 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	sodium azide	natural abundance	0.01 %

13 3D HBHA(CO)NH

Instrument

Home-built OMEGA - 600 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	VARC244	[U-13C; U-15N]	1 mM
10	H2O	natural abundance	95 %
11	D2O	[U-2H]	5 %
12	DTT	natural abundance	1 mM
13	DSS	natural abundance	0.1 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.01 %

14 4D 13C-13C NOESY

Instrument

Home-built OMEGA - 500 MHz Oxford supercon magnets, home built consoles controlled by GE omega computers

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	VARC244	[U-13C; U-15N]	1 mM
18	D2O	[U-2H]	100 %
19	DTT	natural abundance	1 mM
20	DSS	natural abundance	0.1 mM
21	sodium phosphate	natural abundance	20 mM
22	sodium chloride	natural abundance	50 mM
23	sodium azide	natural abundance	0.01 %

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Found 1 Document (4.564 seconds)

BMRB: 16911

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. VARC244, : 1 : 11 entities Detail

Experiments performed 14 experiments Detail

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