NMR Structure of Escherichia coli BamE, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex
GSHMSTLERV VYRPDINQGN YLTANDVSKI RVGMTQQQVA YALGTPLMSD PFGTNTWFYV FRQQPGHEGV TQQTLTLTFN SSGVLTNIDN KPALSGN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 71.0 % (785 of 1106) | 77.2 % (440 of 570) | 58.5 % (250 of 427) | 87.2 % (95 of 109) |
Backbone | 71.0 % (406 of 572) | 88.9 % (176 of 198) | 51.8 % (146 of 282) | 91.3 % (84 of 92) |
Sidechain | 73.5 % (457 of 622) | 71.0 % (264 of 372) | 78.1 % (182 of 233) | 64.7 % (11 of 17) |
Aromatic | 41.3 % (38 of 92) | 41.3 % (19 of 46) | 40.0 % (18 of 45) | 100.0 % (1 of 1) |
Methyl | 89.6 % (95 of 106) | 86.8 % (46 of 53) | 92.5 % (49 of 53) |
1. E. coli lipoprotein
GSHMSTLERV VYRPDINQGN YLTANDVSKI RVGMTQQQVA YALGTPLMSD PFGTNTWFYV FRQQPGHEGV TQQTLTLTFN SSGVLTNIDN KPALSGNSolvent system 85% H2O/15% D2O, Pressure 1 atm, Temperature 298 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | E. coli protein | [U-100% 13C; U-100% 15N] | 0.5 mM | |
2 | D2O | natural abundance | 15 % | |
3 | H2O | natural abundance | 85 % | |
4 | Na2HPO4/NaH2PO4 | natural abundance | 20 mM |