BMRB: 16935

Solution structure of the PECAM-1 cytoplasmic tail with DPC

Authors

Lytle, B.L., Peterson, F.C., Volkman, B.F., Paddock, C., Newman, D.K.

Assembly

PECAM-1

Entity

1. PECAM-1 (polymer, Thiol state: not present), 55 monomers, 5980.301 Da Detail

GSSDVQYTEV QVSSAESHKD LGKKDTETVY SEVRKAVPDA VESRYSRTEG SLDGT

Formula weight

5980.301 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 76.1 %, Completeness (bb): 88.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	76.1 % (446 of 586)	69.9 % (211 of 302)	82.5 % (188 of 228)	83.9 % (47 of 56)
Backbone	88.1 % (289 of 328)	85.8 % (97 of 113)	90.1 % (145 of 161)	87.0 % (47 of 54)
Sidechain	66.7 % (206 of 309)	60.3 % (114 of 189)	78.0 % (92 of 118)	0.0 % (0 of 2)
Aromatic	53.6 % (15 of 28)	71.4 % (10 of 14)	35.7 % (5 of 14)
Methyl	75.0 % (39 of 52)	76.9 % (20 of 26)	73.1 % (19 of 26)

1. PECAM-1

GSSDVQYTEV QVSSAESHKD LGKKDTETVY SEVRKAVPDA VESRYSRTEG SLDGT

Show sample condition

Sample

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0 mM PECAM-1 U-15N/13C, 20 mM sodium phosphate, 50 mM sodium choloride, 2 mM Dithiothreitol, 600 mM dodecylphosphocholine

#	Name	Isotope labeling	Type	Concentration
1	PECAM-1	[U-100% 13C; U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	DTT	natural abundance		2 mM
5	DPC	[U-100% 2H]		600 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

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LACS Plot; CA

Referencing offset: -0.41 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.41 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KY5, Strand ID: A Detail

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Release date

2011-04-11

Citation

Residues within a lipid-associated segment of the PECAM-1 cytoplasmic domain are susceptible to inducible, sequential phosphorylation
Paddock, C., Lytle, B.L., Peterson, F.C., Holyst, T., Newman, P.J., Volkman, B.F., Newman, D.K.
Blood (2011), 117, 6012-6023, PubMed 21464369 , DOI 10.1182/blood-2010-11-317867 , Abstract

Related entities 1. PECAM-1, : 1 : 8 entities Detail

Interaction partners 1. PECAM-1, : 19 interactors Detail

Experiments performed 3 experiments Detail