Chemical shift assignment of CpR82G from Clostridium perfringens. North East Structural Genomics Consortium Target CpR82g.
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 78.6 % (597 of 760) | 74.2 % (288 of 388) | 82.4 % (248 of 301) | 85.9 % (61 of 71) |
Backbone | 90.0 % (367 of 408) | 90.2 % (129 of 143) | 89.8 % (177 of 197) | 89.7 % (61 of 68) |
Sidechain | 68.8 % (284 of 413) | 64.9 % (159 of 245) | 75.8 % (125 of 165) | 0.0 % (0 of 3) |
Aromatic | 28.6 % (20 of 70) | 28.6 % (10 of 35) | 28.6 % (10 of 35) | |
Methyl | 83.8 % (62 of 74) | 81.1 % (30 of 37) | 86.5 % (32 of 37) |
1. CpR82G
MKTGIVNVSS SLNVRSSAST SSKVIGSLSG NTKVTIVGEE GAFYKIEYKG SHGYVAKEYI LEHHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CpR82G | [U-100% 13C; U-100% 15N] | 1.01 mM | |
2 | sodium azide | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 100 mM | |
4 | Calcium Chloride | natural abundance | 5 mM | |
5 | sodium chloride | natural abundance | 100 mM | |
6 | MES | natural abundance | 20 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | CpR82G | U-5% 13C; U-100% 15N | 0.98 mM | |
10 | sodium azide | natural abundance | 0.02 % | |
11 | DTT | natural abundance | 100 mM | |
12 | Calcium Chloride | natural abundance | 5 mM | |
13 | sodium chloride | natural abundance | 100 mM | |
14 | MES | natural abundance | 20 mM | |
15 | H2O | natural abundance | 95 % | |
16 | D2O | natural abundance | 5 % |
Solvent system 82.88% H2O/17.12% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | CpR82G | [U-100% 13C; U-100% 15N] | 0.67 mM | |
18 | sodium azide | natural abundance | 0.016 % | |
19 | DTT | natural abundance | 81.73 mM | |
20 | Calcium Chloride | natural abundance | 4.09 mM | |
21 | sodium chloride | natural abundance | 81.73 mM | |
22 | MES | natural abundance | 16.35 mM | |
23 | C12E5 PEG | natural abundance | 4 % | |
24 | Hexanol | natural abundance | 1.25 % | |
25 | H2O | natural abundance | 82.88 % | |
26 | D2O | natural abundance | 17.12 % |
Solvent system 91.3% H2O/8.7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
27 | CpR82G | natural abundance | 0.92 mM | |
28 | sodium azide | natural abundance | 0.018 % | |
29 | DTT | natural abundance | 91.67 mM | |
30 | Calcium Chloride | natural abundance | 4.58 mM | |
31 | sodium chloride | natural abundance | 91.67 mM | |
32 | MES | natural abundance | 18.32 mM | |
33 | Positively Charged Compressed Polyacrylamide Gel | natural abundance | 7 % | |
34 | H2O | natural abundance | 91.3 % | |
35 | D2O | natural abundance | 8.7 % |