The Structure of RNA Internal Loops with Tandem AG Pairs: 5'GAGC/3'CGAG
Polymer type: polyribonucleotide
Total | 1H | 13C | 31P | |
---|---|---|---|---|
All | 85.5 % (153 of 179) | 97.9 % (93 of 95) | 68.5 % (50 of 73) | 90.9 % (10 of 11) |
Suger, PO4 | 81.1 % (107 of 132) | 97.0 % (64 of 66) | 60.0 % (33 of 55) | 90.9 % (10 of 11) |
Nucleobase | 97.9 % (46 of 47) | 100.0 % (29 of 29) | 94.4 % (17 of 18) | |
Aromatic | 97.6 % (40 of 41) | 100.0 % (23 of 23) | 94.4 % (17 of 18) |
1. RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')
GACGAGCGUC ASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3') | natural abundance | 1 mM | |
2 | sodium chloride | natural abundance | 80 mM | |
3 | sodium phosphate | natural abundance | 10 mM | |
4 | EDTA | natural abundance | 0.5 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3') | natural abundance | 1 mM | |
8 | sodium chloride | natural abundance | 80 mM | |
9 | sodium phosphate | natural abundance | 10 mM | |
10 | EDTA | natural abundance | 0.5 mM | |
11 | D2O | natural abundance | 100 % |