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BMRB: 16969

2XA6

Structural basis for homodimerization of the Src-associated during mitosis, 68 kD protein (Sam68) Qua1 domain

Authors

Meyer, N., Tripsianes, K., Vincendeaux, M., Madl, T., Kateb, F., Brack-werner, R., Sattler, M.

Assembly

Sam68 Qua1 domain

Entity

1. Sam68 Qua1 domain (polymer, Thiol state: not present), 41 monomers, 4605.219 × 2 Da Detail

GAMEPENKYL PELMAEKDSL DPSFTHAMQL LTAEIEKIQK G

Total weight

9210.438 Da

Max. entity weight

4605.219 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

CYANA, ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.7 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.7 % (466 of 482)	98.4 % (250 of 254)	94.1 % (176 of 187)	97.6 % (40 of 41)
Backbone	96.2 % (231 of 240)	98.8 % (80 of 81)	94.2 % (114 of 121)	97.4 % (37 of 38)
Sidechain	97.5 % (274 of 281)	98.3 % (170 of 173)	96.2 % (101 of 105)	100.0 % (3 of 3)
Aromatic	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)
Methyl	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)

1. KH DOMAIN-CONTAINING,RNA-BINDING,SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 1

GAMEPENKYL PELMAEKDSL DPSFTHAMQL LTAEIEKIQK G

Show sample condition

Sample #1

Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

Sample #2

Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 13C, 15N labled Qua1 aligned with Pf-1 filamentous Phages

#	Name	Isotope labeling	Concentration
6	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	NaCl	natural abundance	100 mM
10	potassium phosphate	natural abundance	20 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2XA6, Strand ID: A, B Detail

Release date

2010-07-25

Citation

Structural basis for homodimerization of the Src-associated during mitosis, 68-kDa protein (Sam68) Qua1 domain
Meyer, N., Tripsianes, K., Vincendeau, M., Madl, T., Kateb, F., Brack-Werner, R., Sattler, M.
J. Biol. Chem. (2010), 285, 28893-28901, PubMed 20610388 , DOI 10.1074/jbc.M110.126185 , Abstract

Related entities 1. Sam68 Qua1 domain, : 1 : 2 : 98 entities Detail

Interaction partners 1. Sam68 Qua1 domain, : 102 interactors Detail

More details for 102 interactors identified by 39 citations

Experiments performed 13 experiments Detail

1 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

3 HNCO

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

4 HNCACB

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

5 HNCOCACB

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

6 HCC(H)-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

7 14N/12C-filtered 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

8 14N/12C-filtered 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

9 HNCO

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

10 HSQC (RDC)

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 13C, 15N labled Qua1 aligned with Pf-1 filamentous Phages

#	Name	Isotope labeling	Concentration
6	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	NaCl	natural abundance	100 mM
10	potassium phosphate	natural abundance	20 mM

11 HNCO (RDC)

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
6	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %
9	NaCl	natural abundance	100 mM
10	potassium phosphate	natural abundance	20 mM

12 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

13 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% water, 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 1 mM uniformly 15N, 13C labeled Sam68 Qua1

#	Name	Isotope labeling	Concentration
1	sam68 Qua1	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	potassium phosphate	natural abundance	20 mM

nullKeywords Alternative splicing, Sam68, Qua1, STAR proteins, CD44, structural biology, NMR, homodimer, TRANSCRIPTION, CELL CYCLE;

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Found 1 Document (5.431 seconds)

BMRB: 16969

Sample #1

Sample #2

Related entities 1. Sam68 Qua1 domain, : 1 : 2 : 98 entities Detail

Interaction partners 1. Sam68 Qua1 domain, : 102 interactors Detail

Experiments performed 13 experiments Detail

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