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BMRB: 16983

2KYU

The solution structure of the PHD3 finger of MLL

Authors

Park, S., Bushweller, J.H.

Assembly

PHD3 finger of MLL

Entity

1. PHD3 (polymer, Thiol state: free and other bound), 67 monomers, 7710.561 Da Detail

GSAKGNFCPL CDKCYDDDDY ESKMMQCGKC DRWVHSKCEN LSDEMYEILS NLPESVAYTC VNCTERH

2. ZN (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

7841.379 Da

Max. entity weight

7710.561 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 93.6 %, Completeness (bb): 93.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (701 of 749)	95.1 % (372 of 391)	91.3 % (262 of 287)	94.4 % (67 of 71)
Backbone	93.7 % (373 of 398)	92.6 % (125 of 135)	94.4 % (187 of 198)	93.8 % (61 of 65)
Sidechain	94.5 % (392 of 415)	96.5 % (247 of 256)	90.8 % (139 of 153)	100.0 % (6 of 6)
Aromatic	80.6 % (50 of 62)	90.3 % (28 of 31)	70.0 % (21 of 30)	100.0 % (1 of 1)
Methyl	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)

1. PHD3

GSAKGNFCPL CDKCYDDDDY ESKMMQCGKC DRWVHSKCEN LSDEMYEILS NLPESVAYTC VNCTERH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Temperature 303 K, pH 6.9

#	Name	Isotope labeling	Concentration
1	PHD3	[U-98% 13C; U-98% 15N]	1 mM
2	ZINC ION	natural abundance	100 uM
3	potassium phosphate	natural abundance	25 mM
4	DTT	natural abundance	3 mM
5	sodium chloride	natural abundance	50 mM
6	sodium azide	natural abundance	1 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KYU, Strand ID: A Detail

Release date

2010-08-11

Citation

The PHD3 domain of MLL acts as a CYP33-regulated switch between MLL-mediated activation and repression
Park, S., Osmers, U., Raman, G., Schwantes, R.H., Diaz, M.O., Bushweller, J.H.
Biochemistry (2010), 49, 6576-6586, PubMed 20677832 , DOI 10.1021/bi1009387 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16989 released on 2010-08-11
Title Solution structure of the RRM domain of CYP33

Related entities 1. PHD3, : 1 : 6 : 130 entities Detail

Interaction partners 1. PHD3, : 40 interactors Detail

More details for 40 interactors identified by 18 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC