The solution structure of the PHD3 finger of MLL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.6 % (701 of 749) | 95.1 % (372 of 391) | 91.3 % (262 of 287) | 94.4 % (67 of 71) |
Backbone | 93.7 % (373 of 398) | 92.6 % (125 of 135) | 94.4 % (187 of 198) | 93.8 % (61 of 65) |
Sidechain | 94.5 % (392 of 415) | 96.5 % (247 of 256) | 90.8 % (139 of 153) | 100.0 % (6 of 6) |
Aromatic | 80.6 % (50 of 62) | 90.3 % (28 of 31) | 70.0 % (21 of 30) | 100.0 % (1 of 1) |
Methyl | 100.0 % (40 of 40) | 100.0 % (20 of 20) | 100.0 % (20 of 20) |
1. PHD3
GSAKGNFCPL CDKCYDDDDY ESKMMQCGKC DRWVHSKCEN LSDEMYEILS NLPESVAYTC VNCTERHSolvent system 95% H2O/5% D2O, Temperature 303 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PHD3 | [U-98% 13C; U-98% 15N] | 1 mM | |
2 | ZINC ION | natural abundance | 100 uM | |
3 | potassium phosphate | natural abundance | 25 mM | |
4 | DTT | natural abundance | 3 mM | |
5 | sodium chloride | natural abundance | 50 mM | |
6 | sodium azide | natural abundance | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |