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BMRB: 16992

2QYQ

1H,13C,15N backbone and sidechain assignment of human Raf kinase inhibitor protein from Escherichia coli

Authors

Cuiying, Y.

Assembly

RKIP

Entity

1. RKIP (polymer, Thiol state: all free), 202 monomers, 22966.55 Da Detail

MSYYHHHHHH EGVRTMPVDL SKWSGPLSLQ EVDEQPQHPL HVTYAGAAVD ELGKVLTPTQ VKNRPTSISW DGLDSGKLYT LVLTDPDAPS RKDPKYREWH HFLVVNMKGN DISSGTVLSD YVGSGPPKGT GLHRYVWLVY EQDRPLKCDE PILSNRSGDH RGKFKVASFR KKYELRAPVA GTCYQAEWDD YVPKLYEQLS GK

Formula weight

22966.55 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.6 %, Completeness: 80.5 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.5 % (1906 of 2367)	80.6 % (995 of 1234)	79.2 % (737 of 930)	85.7 % (174 of 203)
Backbone	91.3 % (1079 of 1182)	91.9 % (372 of 405)	90.5 % (534 of 590)	92.5 % (173 of 187)
Sidechain	72.7 % (997 of 1371)	75.2 % (623 of 829)	70.9 % (373 of 526)	6.3 % (1 of 16)
Aromatic	6.8 % (16 of 234)	8.5 % (10 of 117)	5.4 % (6 of 112)	0.0 % (0 of 5)
Methyl	95.8 % (184 of 192)	96.9 % (93 of 96)	94.8 % (91 of 96)

1. RKIP

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 4.0

#	Name	Isotope labeling	Concentration
1	acetic acid	natural abundance	200 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	RKIP	[U-100% 13C; U-100% 15N]	3 mM
5	DTT	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.93 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.93 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.51 ppm, Outliers: 6 Detail

Release date

2010-10-13

Citation

1H, 13C, 15N backbone and side-chain resonance assignments of the human Raf-1 kinase inhibitor protein
Yi, C., Peng, Y., Guo, C., Lin, D.
Biomol. NMR Assign. (2011), 5, 63-66, PubMed 20924725 , DOI 10.1007/s12104-010-9268-z , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17382 released on 2011-02-14
Title Solution structure of the human Raf-1 kinase inhibitor protein

Related entities 1. RKIP, : 1 : 5 : 73 entities Detail

Interaction partners 1. RKIP, : 20 interactors Detail

More details for 20 interactors identified by 10 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC