ASIP(80-132, P103A, P105A, Q115Y, S124Y)
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 85.1 % (257 of 302) | 85.1 % (257 of 302) |
Backbone | 97.0 % (98 of 101) | 97.0 % (98 of 101) |
Sidechain | 79.1 % (159 of 201) | 79.1 % (159 of 201) |
Aromatic | 88.9 % (16 of 18) | 88.9 % (16 of 18) |
Methyl | 100.0 % (24 of 24) | 100.0 % (24 of 24) |
1. ASIP(80-132, P103A, P105A, Q115Y, S124Y)
KKVVRPRTPL SAPCVATRNS CKPAAAACCD PCASCYCRFF RSACYCRVLS LNCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details samples was brought to pH 5 using NaOD
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ASIP(80-132, P103A, P105A, Q115Y, S124Y) | natural abundance | 1-2 mM | |
2 | D2O | natural abundance | 10 % | |
3 | H2O | natural abundance | 90 % | |
4 | acetic acid | [U-100% 2H] | 50 mM | |
5 | sodium azide | natural abundance | .1 % w/v | |
6 | TSP | natural abundance | 200 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details Deuterium Exchange Experiments, samples was brought to pH 4 using NaOD
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | ASIP(80-132, P103A, P105A, Q115Y, S124Y) | natural abundance | 1-2 mM | |
8 | D2O | [U-100% 2H] | 100 % | |
9 | acetic acid | [U-100% 2H] | 50 mM | |
10 | sodium azide | natural abundance | .1 % w/v | |
11 | TSP | natural abundance | 200 uM |