Three-dimensional structure of a truncated phosphoribosylanthranilate isomerase (residues 255-384) from Escherichia coli
MGENKVCGLT RGQDAKAAYD AGAIYGGLIF VATSPRCVNV EQAQEVMAAA PLQYVGVFRN HDIADVVDKA KVLSLAAVQL HGNEEQLYID TLREALPAHV AIWKALSVGE TLPAREFQHV DKYVLDNGQG GAGS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.8 % (1327 of 1477) | 90.1 % (680 of 755) | 88.1 % (509 of 578) | 95.8 % (138 of 144) |
Backbone | 98.4 % (783 of 796) | 97.5 % (270 of 277) | 99.5 % (387 of 389) | 96.9 % (126 of 130) |
Sidechain | 82.8 % (664 of 802) | 85.8 % (410 of 478) | 78.1 % (242 of 310) | 85.7 % (12 of 14) |
Aromatic | 40.8 % (40 of 98) | 77.6 % (38 of 49) | 2.1 % (1 of 48) | 100.0 % (1 of 1) |
Methyl | 94.3 % (166 of 176) | 95.5 % (84 of 88) | 93.2 % (82 of 88) |
1. trPRAI
MGENKVCGLT RGQDAKAAYD AGAIYGGLIF VATSPRCVNV EQAQEVMAAA PLQYVGVFRN HDIADVVDKA KVLSLAAVQL HGNEEQLYID TLREALPAHV AIWKALSVGE TLPAREFQHV DKYVLDNGQG GAGSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | trPRAI | natural abundance | 0.8 mM | |
2 | HEPES | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | DSS | natural abundance | 0.1 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | trPRAI | [U-99% 15N] | 0.6 ~ 1.0 mM | |
9 | HEPES | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 100 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | DSS | natural abundance | 0.1 mM | |
13 | H2O | natural abundance | 95 % | |
14 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | trPRAI | [U-98% 13C; U-98% 15N] | 0.6 ~ 0.8 mM | |
16 | HEPES | natural abundance | 20 mM | |
17 | sodium chloride | natural abundance | 100 mM | |
18 | DTT | natural abundance | 1 mM | |
19 | DSS | natural abundance | 0.1 mM | |
20 | H2O | natural abundance | 95 % | |
21 | D2O | natural abundance | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
22 | trPRAI | [U-98% 13C; U-98% 15N] | 0.7 mM | |
23 | HEPES | natural abundance | 20 mM | |
24 | sodium chloride | natural abundance | 100 mM | |
25 | DTT | natural abundance | 1 mM | |
26 | DSS | natural abundance | 0.1 mM | |
27 | D2O | natural abundance | 100 % |