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BMRB: 17010

6F9K

Backbone Amide relaxation parameters for wild-type Tryptophan Repressor

Authors

Goel, A.

Assembly

Tryptophan apo-repressor

Entity

1. Tryptophan apo-repressor (polymer, Thiol state: not present), 108 monomers, 12355.00 × 2 Da Detail

MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA LGTRVRIVEE LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL EEVLLKSD

Total weight

24710.0 Da

Max. entity weight

12355.0 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters, spectral_density_values

Chem. Shift Complete

Sequence coverage: 86.1 %, Completeness: 32.3 %, Completeness (bb): 58.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	32.3 % (414 of 1281)	19.5 % (131 of 672)	39.1 % (192 of 491)	77.1 % (91 of 118)
Backbone	58.3 % (373 of 640)	45.2 % (98 of 217)	58.0 % (185 of 319)	86.5 % (90 of 104)
Sidechain	16.5 % (123 of 744)	7.3 % (33 of 455)	32.4 % (89 of 275)	7.1 % (1 of 14)
Aromatic	0.0 % (0 of 58)	0.0 % (0 of 29)	0.0 % (0 of 27)	0.0 % (0 of 2)
Methyl	14.0 % (19 of 136)	10.3 % (7 of 68)	17.6 % (12 of 68)

1. apo-TrpR

MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA LGTRVRIVEE LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL EEVLLKSD

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 5.7, Details All samples had the same conditions

#	Name	Isotope labeling	Concentration
1	H2O	natural abundance	95 %
2	D2O	[U-100% 2H]	5 %
3	PMSF	natural abundance	0.1 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium azide	natural abundance	0.01 %
6	sodium chloride	natural abundance	500 mM
7	EDTA	natural abundance	1 mM
8	apo-WT-TrpR	[U-100% 13C; U-100% 15N]	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.71 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.71 ppm, Outliers: 1 Detail

Heteronucl. T₁

72 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

Heteronucl. T₂

72 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

Heteronucl. NOE

72 NOE values in 1 lists
Value type peak height, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

Heteronucl. T₁/T₂

72 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

Order parameters

61 S² values in 1 lists
Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

Spectral density

62 values in 1 lists
Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

Release date

2010-07-08

Citation 1

Backbone amide dynamics studies of Apo-L75F-TrpR, a temperature-sensitive mutant of the tryptophan repressor protein (TrpR): comparison with the (15)N NMR relaxation profiles of wild-type and A77V mutant Apo-TrpR repressors
Goel, A., Tripet, B.P., Tyler, R.C., Nebert, L.D., Copie, V.
Biochemistry (2010), 49, 8006-8019, PubMed 20718459 , DOI 10.1021/bi100508u , Abstract

Show more 1 citaion

Citation 2

Three-dimensional solution NMR structure of Apo-L75F-TrpR, a temperature-sensitive mutant of the tryptophan repressor protein
Tyler, R.
Biochemistry (2002), 41, 11954-11962, PubMed 12356295 , Abstract

Hide non-primary 1 citaion

Related entities 1. Tryptophan apo-repressor, : 1 : 6 : 2 : 22 : 38 entities Detail

Interaction partners 1. Tryptophan apo-repressor, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC