BMRB: 17012

Backbone Amide relaxation parameters for mutant L75F Tryptophan Repressor

Authors

Goel, A.

Assembly

Tryptophan apo-repressor

Entity

1. Tryptophan apo-repressor (polymer, Thiol state: not present), 108 monomers, 12389.02 × 2 Da Detail

MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA LGTRVRIVEE LLRGEMSQRE LKNEFGAGIA TITRGSNSLK AAPVELRQWL EEVLLKSD

Total weight

24778.04 Da

Max. entity weight

12389.02 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters, spectral_density_values

Heteronucl. T₁

92 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 318 K, pH 5.7

Experiment name 2D 15N T1

Heteronucl. T₂

92 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 318 K, pH 5.7

Experiment name 2D 15N T2 interleaved

Heteronucl. NOE

92 NOE values in 1 lists
Value type peak height, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 318 K, pH 5.7

Experiment name 2D 15N {1H} nOe

Heteronucl. T₁/T₂

92 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 318 K, pH 5.7

Order parameters

88 S² values in 1 lists
Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 318 K, pH 5.7

Experiment name 2D 15N T1, 2D 15N {1H} nOe, 2D 15N T2 interleaved

List #1 order_parameter_apoL75F

Spectral density

92 values in 1 lists
Pressure 1 atm, Temperature 318 K, pH 5.7 Detail

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Pressure 1 atm, Temperature 318 K, pH 5.7

Experiment name 2D 15N T1, 2D 15N {1H} nOe, 2D 15N T2 interleaved

Release date

2010-07-08

Citation 1

Backbone amide dynamics studies of Apo-L75F-TrpR, a temperature-sensitive mutant of the tryptophan repressor protein (TrpR): comparison with the (15)N NMR relaxation profiles of wild-type and A77V mutant Apo-TrpR repressors
Goel, A., Tripet, B.P., Tyler, R.C., Nebert, L.D., Copie, V.
Biochemistry (2010), 49, 8006-8019, PubMed 20718459 , DOI 10.1021/bi100508u , Abstract

Show more 1 citaion

Citation 2

Three-dimensional solution NMR structure of Apo-L75F-TrpR, a temperature-sensitive mutant of the tryptophan repressor protein
Tyler, R.
Biochemistry (2002), 41, 11954-11962, PubMed 12356295 , Abstract

Hide non-primary 1 citaion

Related entities 1. Tryptophan apo-repressor, : 1 : 28 : 40 entities Detail

Interaction partners 1. Tryptophan apo-repressor, : 2 interactors Detail

Experiments performed 4 experiments Detail