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Found 1 Document (2.728 seconds)

BMRB: 17020

2KZV

Solution NMR structure of CV_0373(175-257) protein from Chromobacterium violaceum, Northeast Structural Genomics Consortium Target CvR118A

Authors

Yang, Y., Ramelot, T.A., Wang, D., Ciccosanti, C., Mao, L., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

CV 0373(175-257) protein

Entity

1. CV 0373(175-257) protein (polymer, Thiol state: not present), 92 monomers, 10579.69 Da Detail

MPHKEKHPLQ DMFTSAIEAV ARDSGWAELS AVGSYLAKND PSFDPRNWGH GRLSQMVKKL DFLTVQESRN GSKLHSEIRL RHDGLEHHHH HH

Formula weight

10579.69 Da

Source organism

Chromobacterium violaceum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.7 % (997 of 1076)	93.1 % (524 of 563)	91.1 % (380 of 417)	96.9 % (93 of 96)
Backbone	95.8 % (521 of 544)	96.8 % (180 of 186)	94.8 % (256 of 270)	96.6 % (85 of 88)
Sidechain	89.3 % (552 of 618)	90.2 % (340 of 377)	87.6 % (204 of 233)	100.0 % (8 of 8)
Aromatic	54.7 % (58 of 106)	54.7 % (29 of 53)	52.9 % (27 of 51)	100.0 % (2 of 2)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. CV 0373(175-257) protein

MPHKEKHPLQ DMFTSAIEAV ARDSGWAELS AVGSYLAKND PSFDPRNWGH GRLSQMVKKL DFLTVQESRN GSKLHSEIRL RHDGLEHHHH HH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	CV_0373(175-257) protein	[U-5% 13C; U-100% 15N]	0.82 (±0.08) mM
8	ammonium acetate	natural abundance	20 (±1.0) mM
9	sodium chloride	natural abundance	100 (±5.0) mM
10	calcium chloride	natural abundance	5 (±0.2) mM
11	DTT	natural abundance	10 (±0.5) mM
12	sodium azide	natural abundance	0.02 (±0.001) %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
14	ammonium acetate	natural abundance	20 (±1.0) mM
15	sodium chloride	natural abundance	100 (±5.0) mM
16	calcium chloride	natural abundance	5 (±0.2) mM
17	DTT	natural abundance	10 (±0.5) mM
18	sodium azide	natural abundance	0.02 (±0.001) %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KZV, Strand ID: A Detail

Release date

2012-08-01

Citation

Solution NMR structure of BT_0084, a conjugative transposon lipoprotein from Bacteroides thetaiotamicron
Ramelot, T.A., Yang, Y., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Proteins (2012), 80, 667-670, PubMed 22116783 , DOI 10.1002/prot.23235 , Abstract

Related entities 1. CV 0373(175-257) protein, : 1 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

2 2D 1H-13C HSQC_CT

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
7	CV_0373(175-257) protein	[U-5% 13C; U-100% 15N]	0.82 (±0.08) mM
8	ammonium acetate	natural abundance	20 (±1.0) mM
9	sodium chloride	natural abundance	100 (±5.0) mM
10	calcium chloride	natural abundance	5 (±0.2) mM
11	DTT	natural abundance	10 (±0.5) mM
12	sodium azide	natural abundance	0.02 (±0.001) %

3 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

4 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

5 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

6 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

7 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

8 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

9 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

10 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

11 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

12 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

13 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

14 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

15 3D (H)CCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
14	ammonium acetate	natural abundance	20 (±1.0) mM
15	sodium chloride	natural abundance	100 (±5.0) mM
16	calcium chloride	natural abundance	5 (±0.2) mM
17	DTT	natural abundance	10 (±0.5) mM
18	sodium azide	natural abundance	0.02 (±0.001) %

16 4D CC NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
13	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
14	ammonium acetate	natural abundance	20 (±1.0) mM
15	sodium chloride	natural abundance	100 (±5.0) mM
16	calcium chloride	natural abundance	5 (±0.2) mM
17	DTT	natural abundance	10 (±0.5) mM
18	sodium azide	natural abundance	0.02 (±0.001) %

17 2D 1H-15N HSQC_swN150ppm

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_0373(175-257) protein	[U-100% 13C; U-100% 15N]	0.9 (±0.09) mM
2	ammonium acetate	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	calcium chloride	natural abundance	5 (±0.2) mM
5	DTT	natural abundance	10 (±0.5) mM
6	sodium azide	natural abundance	0.02 (±0.001) %

nullKeywords Chromobacterium violaceum, CV_0373(175-257) protein, Northeast Structural Genomics Consortium (NESG), Solution NMR, NMR solution structure

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Found 1 Document (2.728 seconds)

BMRB: 17020

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CV 0373(175-257) protein, : 1 entities Detail

Experiments performed 17 experiments Detail

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