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Solution NMR structure of CV_0373(175-257) protein from Chromobacterium violaceum, Northeast Structural Genomics Consortium Target CvR118A
Authors
Yang, Y., Ramelot, T.A., Wang, D., Ciccosanti, C., Mao, L., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Assembly
CV 0373(175-257) protein
Entity
1. CV 0373(175-257) protein (polymer, Thiol state: not present), 92 monomers, 10579.69 Da Detail

MPHKEKHPLQ DMFTSAIEAV ARDSGWAELS AVGSYLAKND PSFDPRNWGH GRLSQMVKKL DFLTVQESRN GSKLHSEIRL RHDGLEHHHH HH


Formula weight
10579.69 Da
Source organism
Chromobacterium violaceum
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.7 % (997 of 1076)93.1 % (524 of 563)91.1 % (380 of 417)96.9 % (93 of 96)
Backbone95.8 % (521 of 544)96.8 % (180 of 186)94.8 % (256 of 270)96.6 % (85 of 88)
Sidechain89.3 % (552 of 618)90.2 % (340 of 377)87.6 % (204 of 233)100.0 % (8 of 8)
Aromatic54.7 % (58 of 106)54.7 % (29 of 53)52.9 % (27 of 51)100.0 % (2 of 2)
Methyl100.0 % (76 of 76)100.0 % (38 of 38)100.0 % (38 of 38)

1. CV 0373(175-257) protein

MPHKEKHPLQ DMFTSAIEAV ARDSGWAELS AVGSYLAKND PSFDPRNWGH GRLSQMVKKL DFLTVQESRN GSKLHSEIRL RHDGLEHHHH HH

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)


#NameIsotope labelingTypeConcentration
1CV_0373(175-257) protein[U-100% 13C; U-100% 15N]0.9 (±0.09) mM
2ammonium acetatenatural abundance20 (±1.0) mM
3sodium chloridenatural abundance100 (±5.0) mM
4calcium chloridenatural abundance5 (±0.2) mM
5DTTnatural abundance10 (±0.5) mM
6sodium azidenatural abundance0.02 (±0.001) %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)


#NameIsotope labelingTypeConcentration
7CV_0373(175-257) protein[U-5% 13C; U-100% 15N]0.82 (±0.08) mM
8ammonium acetatenatural abundance20 (±1.0) mM
9sodium chloridenatural abundance100 (±5.0) mM
10calcium chloridenatural abundance5 (±0.2) mM
11DTTnatural abundance10 (±0.5) mM
12sodium azidenatural abundance0.02 (±0.001) %
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.1)


#NameIsotope labelingTypeConcentration
13CV_0373(175-257) protein[U-100% 13C; U-100% 15N]0.9 (±0.09) mM
14ammonium acetatenatural abundance20 (±1.0) mM
15sodium chloridenatural abundance100 (±5.0) mM
16calcium chloridenatural abundance5 (±0.2) mM
17DTTnatural abundance10 (±0.5) mM
18sodium azidenatural abundance0.02 (±0.001) %

LACS Plot; CA
Referencing offset: -0.23 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.23 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.11 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2KZV, Strand ID: A Detail


Release date
2012-08-01
Citation
Solution NMR structure of BT_0084, a conjugative transposon lipoprotein from Bacteroides thetaiotamicron
Ramelot, T.A., Yang, Y., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Proteins (2012), 80, 667-670, PubMed 22116783 , DOI 10.1002/prot.23235 ,
Related entities 1. CV 0373(175-257) protein, : 1 entities Detail
Experiments performed 17 experiments Detail
nullKeywords Chromobacterium violaceum, CV_0373(175-257) protein, Northeast Structural Genomics Consortium (NESG), Solution NMR, NMR solution structure