Solution NMR structure of the ZNF216 A20 zinc finger
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS16:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS20:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS32:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS35:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.2 % (524 of 653) | 73.3 % (252 of 344) | 85.1 % (206 of 242) | 98.5 % (66 of 67) |
Backbone | 93.6 % (339 of 362) | 85.2 % (109 of 128) | 98.3 % (174 of 177) | 98.2 % (56 of 57) |
Sidechain | 69.2 % (238 of 344) | 66.2 % (143 of 216) | 72.0 % (85 of 118) | 100.0 % (10 of 10) |
Aromatic | 50.0 % (15 of 30) | 100.0 % (15 of 15) | 0.0 % (0 of 15) | |
Methyl | 92.9 % (26 of 28) | 92.9 % (13 of 14) | 92.9 % (13 of 14) |
1. ZNF216-A20
GSMAQETNQT PGPMLCSTGC GFYGNPRTNG MCSVCYKEHL QRQQNSGRMS PMGTASGSNS PTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details 50uM ZnCl2 used 0.1 mM DSS used for referencing
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ZNF216-A20 | natural abundance | 1 (±0.1) mM | |
2 | ZnCl2 | natural abundance | 50 (±0.2) uM | |
3 | DSS | natural abundance | 0.1 (±0.01) mM | |
4 | TRIS | natural abundance | 5 (±0.5) mM | |
5 | sodium chloride | natural abundance | 50 (±5.0) mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details 50 uM ZnCl2 added 0.1 mM DSS added
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | ZNF216-A20 | [U-100% 15N] | 1.2 (±0.1) mM | |
9 | ZnCl2 | natural abundance | 50 (±0.2) uM | |
10 | DSS | natural abundance | 0.1 (±0.01) mM | |
11 | TRIS | natural abundance | 5 (±0.5) mM | |
12 | sodium chloride | natural abundance | 50 (±5.0) mM | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details 50 uM ZnCl2 added 0.1 mM DSS used
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | ZNF216-A20 | [U-100% 13C; U-100% 15N] | 0.8 (±0.2) mM | |
16 | ZnCl2 | natural abundance | 50 (±0.2) uM | |
17 | DSS | natural abundance | 0.1 (±0.01) mM | |
18 | TRIS | natural abundance | 5 (±0.5) mM | |
19 | sodium chloride | natural abundance | 50 (±5.0) mM | |
20 | H2O | natural abundance | 90 % | |
21 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details Sample 2 soaked in 7% PAG gel
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
22 | ZNF216-A20 | [U-100% 15N] | 1.2 (±0.1) mM | |
23 | ZnCl2 | natural abundance | 50 (±0.2) uM | |
24 | DSS | natural abundance | 0.1 (±0.01) mM | |
25 | TRIS | natural abundance | 5 (±0.5) mM | |
26 | sodium chloride | natural abundance | 50 (±5.0) mM | |
27 | Polyacrylamide gel | natural abundance | 7 (±0.7) % | |
28 | H2O | natural abundance | 90 % | |
29 | D2O | natural abundance | 10 % |