Solution NMR Structure of protein BT2368 from Bacteroides thetaiotaomicron, Northeast Structural Genomics Consortium Target BtR375
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.0 % (868 of 954) | 91.9 % (451 of 491) | 89.3 % (334 of 374) | 93.3 % (83 of 89) |
Backbone | 93.3 % (457 of 490) | 92.9 % (158 of 170) | 93.7 % (224 of 239) | 92.6 % (75 of 81) |
Sidechain | 89.2 % (481 of 539) | 91.3 % (293 of 321) | 85.7 % (180 of 210) | 100.0 % (8 of 8) |
Aromatic | 57.4 % (54 of 94) | 66.0 % (31 of 47) | 46.7 % (21 of 45) | 100.0 % (2 of 2) |
Methyl | 99.0 % (97 of 98) | 100.0 % (49 of 49) | 98.0 % (48 of 49) |
1. BtR375
MDKKIVGANA GKVWHALNEA DGISIPELAR KVNLSVESTA LAVGWLAREN KVVIERKNGL IEIYNEGHFD FSFGLEHHHH HHSolvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.5, Details 1.3 mM [U-100% 13C; U-100% 15N] BtR375, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BtR375 | [U-100% 13C; U-100% 15N] | 1.1 mM | |
2 | ammonium acetate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | sodium azide | natural abundance | 0.02 % | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.5, Details 1.3 mM [U-5% 13C; U-100% 15N] BtR375, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | BtR375 | [U-5% 13C; U-100% 15N] | 1.1 mM | |
9 | ammonium acetate | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 100 mM | |
11 | calcium chloride | natural abundance | 5 mM | |
12 | sodium azide | natural abundance | 0.02 % | |
13 | H2O | natural abundance | 90 % | |
14 | D2O | natural abundance | 10 % |
Solvent system 80% H2O/20% D2O, Pressure 1 null, Temperature 298 K, pH 6.5, Details 1.3 mM [U-5% 13C; U-100% 15N] BtR375, 80% H2O/20% D2O, aligned in PEG/hexanol
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | BtR375 | [U-5% 13C; U-100% 15N] | 0.9 mM | |
16 | ammonium acetate | natural abundance | 18 mM | |
17 | sodium chloride | natural abundance | 91 mM | |
18 | calcium chloride | natural abundance | 4.6 mM | |
19 | sodium azide | natural abundance | 0.018 % | |
20 | C12E5 polyethylene glycol | natural abundance | 4 % | |
21 | hexanol | natural abundance | 4 % | |
22 | H2O | natural abundance | 80 % | |
23 | D2O | natural abundance | 20 % |
Solvent system 80% H2O/20% D2O, Pressure 1 null, Temperature 298 K, pH 6.5, Details 1.3 mM [U-5% 13C; U-100% 15N] BtR375, 80% H2O/20% D2O, aligned in polyacrylamide gel
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
24 | BtR375 | [U-5% 13C; U-100% 15N] | 1.2 mM | |
25 | ammonium acetate | natural abundance | 18 mM | |
26 | sodium chloride | natural abundance | 91 mM | |
27 | calcium chloride | natural abundance | 4.6 mM | |
28 | sodium azide | natural abundance | 0.018 % | |
29 | H2O | natural abundance | 80 % | |
30 | D2O | natural abundance | 20 % |