NMR-derived spatial structure of water-soluble Lynx1
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS4:SG | 1:CYS27:SG |
2 | disulfide | sing | 1:CYS7:SG | 1:CYS14:SG |
3 | disulfide | sing | 1:CYS20:SG | 1:CYS45:SG |
4 | disulfide | sing | 1:CYS49:SG | 1:CYS66:SG |
5 | disulfide | sing | 1:CYS67:SG | 1:CYS72:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 65.2 % (533 of 818) | 94.8 % (399 of 421) | 18.6 % (60 of 322) | 98.7 % (74 of 75) |
Backbone | 56.4 % (246 of 436) | 98.0 % (144 of 147) | 15.1 % (33 of 219) | 98.6 % (69 of 70) |
Sidechain | 67.5 % (306 of 453) | 93.1 % (255 of 274) | 26.4 % (46 of 174) | 100.0 % (5 of 5) |
Aromatic | 45.2 % (38 of 84) | 90.5 % (38 of 42) | 0.0 % (0 of 42) | |
Methyl | 96.2 % (50 of 52) | 100.0 % (26 of 26) | 92.3 % (24 of 26) |
1. Lynx1
MLDCHVCAYN GDNCFNPMRC PAMVAYCMTT RTYYTPTRMK VSKSCVPRCF ETVYDGYSKH ASTTSCCQYD LCNGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.3 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Lynx1 | [U-99% 15N] | 0.5 mM | |
2 | acetic acid | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 1 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.3 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Lynx1 | natural abundance | 1.0 mM | |
7 | acetic acid | natural abundance | 20 mM | |
8 | sodium azide | natural abundance | 1 mM | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % |