BMRBj - BMREntryMaster

Found 1 Document (0.774 seconds)

BMRB: 17028

2L04

The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein

Authors

Pell, L.G., Gasmi-Seabrook, G.M.C., Donaldson, L.W., Howell, P., Davidson, A.R., Maxwell, K.L.

Assembly

Bacteriophage Lambda Tail Tube-gpV-C

Entity

1. Bacteriophage Lambda Tail Tube-gpV-C (polymer, Thiol state: not present), 91 monomers, 8875.969 Da Detail

GAMAAATGMT VTPASTSVVK GQSTTLTVAF QPEGVTDKSF RAVSADKTKA TVSVSGMTIT VNGVAAGKVN IPVVSGNGEF AAVAEITVTA S

Formula weight

8875.969 Da

Source organism

Escherichia virus Lambda

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.5 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.5 % (876 of 917)	96.0 % (437 of 455)	94.0 % (347 of 369)	98.9 % (92 of 93)
Backbone	97.4 % (526 of 540)	98.9 % (186 of 188)	95.8 % (253 of 264)	98.9 % (87 of 88)
Sidechain	94.1 % (432 of 459)	94.4 % (252 of 267)	93.6 % (175 of 187)	100.0 % (5 of 5)
Aromatic	60.0 % (18 of 30)	66.7 % (10 of 15)	53.3 % (8 of 15)
Methyl	95.5 % (128 of 134)	95.5 % (64 of 67)	95.5 % (64 of 67)

1. gpV-C

GAMAAATGMT VTPASTSVVK GQSTTLTVAF QPEGVTDKSF RAVSADKTKA TVSVSGMTIT VNGVAAGKVN IPVVSGNGEF AAVAEITVTA S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	200 mM
8	D20	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.68 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L04, Strand ID: A Detail

Release date

2010-09-23

Citation

The solution structure of the C-terminal Ig-like domain of the bacteriophage λ tail tube protein
Pell, L.G., Gasmi-Seabrook, G.M.C., Morais, M., Neudecker, P., Kanelis, V., Bona, D., Donaldson, L.W., Edwards, A.M., Howell, P., Davidson, A.R., Maxwell, K.L.
J. Mol. Biol. (2010), 403, 468-479, PubMed 20826161 , DOI 10.1016/j.jmb.2010.08.044 , Abstract

Related entities 1. Bacteriophage Lambda Tail Tube-gpV-C, : 1 : 1 : 8 entities Detail

Interaction partners 1. Bacteriophage Lambda Tail Tube-gpV-C, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

3 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

4 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	200 mM
8	D20	natural abundance	100 %

5 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

6 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

8 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	200 mM
8	D20	natural abundance	100 %

9 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

nullKeywords bacteriophage lambda, gpV, phage tail, Ig-like domain

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Found 1 Document (0.774 seconds)

BMRB: 17028

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Bacteriophage Lambda Tail Tube-gpV-C, : 1 : 1 : 8 entities Detail

Interaction partners 1. Bacteriophage Lambda Tail Tube-gpV-C, : 6 interactors Detail

Experiments performed 9 experiments Detail

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