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The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein
Authors
Pell, L.G., Gasmi-Seabrook, G.M.C., Donaldson, L.W., Howell, P., Davidson, A.R., Maxwell, K.L.
Assembly
Bacteriophage Lambda Tail Tube-gpV-C
Entity
1. Bacteriophage Lambda Tail Tube-gpV-C (polymer, Thiol state: not present), 91 monomers, 8875.969 Da Detail

GAMAAATGMT VTPASTSVVK GQSTTLTVAF QPEGVTDKSF RAVSADKTKA TVSVSGMTIT VNGVAAGKVN IPVVSGNGEF AAVAEITVTA S


Formula weight
8875.969 Da
Source organism
Escherichia virus Lambda
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 95.5 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All95.5 % (876 of 917)96.0 % (437 of 455)94.0 % (347 of 369)98.9 % (92 of 93)
Backbone97.4 % (526 of 540)98.9 % (186 of 188)95.8 % (253 of 264)98.9 % (87 of 88)
Sidechain94.1 % (432 of 459)94.4 % (252 of 267)93.6 % (175 of 187)100.0 % (5 of 5)
Aromatic60.0 % (18 of 30)66.7 % (10 of 15)53.3 % (8 of 15)
Methyl95.5 % (128 of 134)95.5 % (64 of 67)95.5 % (64 of 67)

1. gpV-C

GAMAAATGMT VTPASTSVVK GQSTTLTVAF QPEGVTDKSF RAVSADKTKA TVSVSGMTIT VNGVAAGKVN IPVVSGNGEF AAVAEITVTA S

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
1gpV-C15N, 13C0.71 ~ 2.5 mM
2sodium phosphatenatural abundance50 mM
3sodium chloridenatural abundance200 mM
4H20natural abundance90 %
5D20natural abundance10 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
6sodium phosphatenatural abundance50 mM
7sodium chloridenatural abundance200 mM
8D20natural abundance100 %

LACS Plot; CA
Referencing offset: 0.22 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.22 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.19 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.68 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2L04, Strand ID: A Detail


Release date
2010-09-23
Citation
The solution structure of the C-terminal Ig-like domain of the bacteriophage λ tail tube protein
Pell, L.G., Gasmi-Seabrook, G.M.C., Morais, M., Neudecker, P., Kanelis, V., Bona, D., Donaldson, L.W., Edwards, A.M., Howell, P., Davidson, A.R., Maxwell, K.L.
J. Mol. Biol. (2010), 403, 468-479, PubMed 20826161 , DOI 10.1016/j.jmb.2010.08.044 ,
Related entities 1. Bacteriophage Lambda Tail Tube-gpV-C, : 1 : 1 : 8 entities Detail
Interaction partners 1. Bacteriophage Lambda Tail Tube-gpV-C, : 6 interactors Detail
Experiments performed 9 experiments Detail
nullKeywords bacteriophage lambda, gpV, phage tail, Ig-like domain