BMRB: 17035

NMR Solution Structure of asr4154 from Nostoc sp. PCC7120 Northeast Structural Genomics Consortium target ID Nsr143

Authors

Feldmann, E.A., Yang, Y.A., Ramelot, T.A., Cort, J.R., Janjua, H., Ciccosanti, C.A., Lee, D., Acton, T.B., Xiao, R.A., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

asr4154

Entity

1. asr4154 (polymer, Thiol state: not present), 62 monomers, 7352.225 Da Detail

MNLRWTSEAK TKLKNIPFFA RSQAKARIEQ LARQAEQDIV TPELVEQARL EFGQLEHHHH HH

Formula weight

7352.225 Da

Source organism

Nostoc sp. PCC 7120 = FACHB-418

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 90.3 %, Completeness: 86.2 %, Completeness (bb): 87.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.2 % (658 of 763)	87.1 % (350 of 402)	84.2 % (246 of 292)	89.9 % (62 of 69)
Backbone	87.0 % (320 of 368)	87.8 % (108 of 123)	85.9 % (159 of 185)	88.3 % (53 of 60)
Sidechain	85.7 % (391 of 456)	86.7 % (242 of 279)	83.3 % (140 of 168)	100.0 % (9 of 9)
Aromatic	54.5 % (36 of 66)	54.5 % (18 of 33)	53.1 % (17 of 32)	100.0 % (1 of 1)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. Hypothetical protein asr4154 from Nostoc sp. PCC7120

MNLRWTSEAK TKLKNIPFFA RSQAKARIEQ LARQAEQDIV TPELVEQARL EFGQLEHHHH HH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical protein asr4154 from Nostoc sp. PCC7120	[U-100% 13C; U-100% 15N]		0.9 (±0.09) mM
2	MES	natural abundance		20 (±1.0) mM
3	sodium chloride	natural abundance		100 (±5.0) mM
4	calcium chloride	natural abundance		5 (±0.2) mM
5	DTT	natural abundance		10 (±0.5) mM
6	sodium azide	natural abundance		0.02 (±0.001) %
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
9	Hypothetical protein asr4154 from Nostoc sp. PCC7120	[U-5% 13C; U-100% 15N]		0.82 (±0.08) mM
10	MES	natural abundance		20 (±1.0) mM
11	sodium chloride	natural abundance		100 (±5.0) mM
12	calcium chloride	natural abundance		5 (±0.2) mM
13	DTT	natural abundance		10 (±0.5) mM
14	sodium azide	natural abundance		0.02 (±0.001) %
15	H2O	natural abundance		90 %
16	D2O	natural abundance		10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
17	Hypothetical protein asr4154 from Nostoc sp. PCC7120	[U-100% 13C; U-100% 15N]		0.9 (±0.09) mM
18	MES	natural abundance		20 (±1.0) mM
19	sodium chloride	natural abundance		100 (±5.0) mM
20	calcium chloride	natural abundance		5 (±0.2) mM
21	DTT	natural abundance		10 (±0.5) mM
22	sodium azide	natural abundance		0.02 (±0.001) %
23	D2O	natural abundance		100 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L09, Strand ID: A Detail

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Release date

2011-12-14

Citation

NMR Solution Structure of asr4154 from Nostoc sp. PCC7120 Northeast Structural Genomics target ID Nsr143
Feldmann, E.A., Yang, Y.A., Ramelot, T.A., Cort, J.R., Janjua, H., Ciccosanti, C.A., Lee, D., Acton, T.B., Xiao, R.A., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Not known

Related entities 1. asr4154, : 1 : 60 entities Detail

Experiments performed 17 experiments Detail