NMR Solution Structure of asr4154 from Nostoc sp. PCC7120 Northeast Structural Genomics Consortium target ID Nsr143
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.2 % (658 of 763) | 87.1 % (350 of 402) | 84.2 % (246 of 292) | 89.9 % (62 of 69) |
Backbone | 87.0 % (320 of 368) | 87.8 % (108 of 123) | 85.9 % (159 of 185) | 88.3 % (53 of 60) |
Sidechain | 85.7 % (391 of 456) | 86.7 % (242 of 279) | 83.3 % (140 of 168) | 100.0 % (9 of 9) |
Aromatic | 54.5 % (36 of 66) | 54.5 % (18 of 33) | 53.1 % (17 of 32) | 100.0 % (1 of 1) |
Methyl | 100.0 % (64 of 64) | 100.0 % (32 of 32) | 100.0 % (32 of 32) |
1. Hypothetical protein asr4154 from Nostoc sp. PCC7120
MNLRWTSEAK TKLKNIPFFA RSQAKARIEQ LARQAEQDIV TPELVEQARL EFGQLEHHHH HHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Hypothetical protein asr4154 from Nostoc sp. PCC7120 | [U-100% 13C; U-100% 15N] | 0.9 (±0.09) mM | |
2 | MES | natural abundance | 20 (±1.0) mM | |
3 | sodium chloride | natural abundance | 100 (±5.0) mM | |
4 | calcium chloride | natural abundance | 5 (±0.2) mM | |
5 | DTT | natural abundance | 10 (±0.5) mM | |
6 | sodium azide | natural abundance | 0.02 (±0.001) % | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Hypothetical protein asr4154 from Nostoc sp. PCC7120 | [U-5% 13C; U-100% 15N] | 0.82 (±0.08) mM | |
10 | MES | natural abundance | 20 (±1.0) mM | |
11 | sodium chloride | natural abundance | 100 (±5.0) mM | |
12 | calcium chloride | natural abundance | 5 (±0.2) mM | |
13 | DTT | natural abundance | 10 (±0.5) mM | |
14 | sodium azide | natural abundance | 0.02 (±0.001) % | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | Hypothetical protein asr4154 from Nostoc sp. PCC7120 | [U-100% 13C; U-100% 15N] | 0.9 (±0.09) mM | |
18 | MES | natural abundance | 20 (±1.0) mM | |
19 | sodium chloride | natural abundance | 100 (±5.0) mM | |
20 | calcium chloride | natural abundance | 5 (±0.2) mM | |
21 | DTT | natural abundance | 10 (±0.5) mM | |
22 | sodium azide | natural abundance | 0.02 (±0.001) % | |
23 | D2O | natural abundance | 100 % |