BMRB: 17062

Solution Structure of Human Plasminogen Kringle 3

Authors

Christen, M.T., Frank, P., Schaller, J., Llinas, M.

Assembly

hPgn rK3

Entity

1. hPgn rK3 (polymer, Thiol state: all disulfide bound), 83 monomers, 9511.310 Da Detail

TYQCLKGTGE NYRGNVAVTV SGHTCQHWSA QTPHTHNRTP ENFPSKNLDE NYCRNPDGKR APWCHTTNSQ VRWEYCKIPS CDS

Formula weight

9511.31 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS81:SG
2	disulfide	sing	1:CYS25:SG	1:CYS64:SG
3	disulfide	sing	1:CYS53:SG	1:CYS76:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.8 %, Completeness: 92.8 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	92.8 % (543 of 585)	91.9 % (453 of 493)	97.8 % (90 of 92)
Backbone	96.3 % (233 of 242)	95.8 % (158 of 165)	97.4 % (75 of 77)
Sidechain	90.4 % (310 of 343)	89.9 % (295 of 328)	100.0 % (15 of 15)
Aromatic	94.2 % (49 of 52)	93.9 % (46 of 49)	100.0 % (3 of 3)
Methyl	100.0 % (26 of 26)	100.0 % (26 of 26)

1. hPgn rK3

TYQCLKGTGE NYRGNVAVTV SGHTCQHWSA QTPHTHNRTP ENFPSKNLDE NYCRNPDGKR APWCHTTNSQ VRWEYCKIPS CDS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, Details 0.02% sodium azide was added to prevent microbial growth

#	Name	Isotope labeling	Type	Concentration
1	hPgn_rK3	[U-97% 15N]		1.0 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		90 %
4	sodium azide	natural abundance		0.02 %

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Chem. Shift Complete2

Sequence coverage: 84.3 %, Completeness: 64.1 %, Completeness (bb): 77.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	64.1 % (750 of 1170)	58.4 % (576 of 986)	94.6 % (174 of 184)
Backbone	77.9 % (377 of 484)	70.6 % (233 of 330)	93.5 % (144 of 154)
Sidechain	54.4 % (373 of 686)	52.3 % (343 of 656)	100.0 % (30 of 30)
Aromatic	56.7 % (59 of 104)	54.1 % (53 of 98)	100.0 % (6 of 6)
Methyl	57.7 % (30 of 52)	57.7 % (30 of 52)

1. hPgn rK3

TYQCLKGTGE NYRGNVAVTV SGHTCQHWSA QTPHTHNRTP ENFPSKNLDE NYCRNPDGKR APWCHTTNSQ VRWEYCKIPS CDS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, Details 0.02% sodium azide was added to prevent microbial growth

#	Name	Isotope labeling	Type	Concentration
1	hPgn_rK3	[U-97% 15N]		1.0 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		90 %
4	sodium azide	natural abundance		0.02 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L0S, Strand ID: A Detail

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Release date

2010-08-18

Citation

Human plasminogen kringle 3: solution structure, functional insights, phylogenetic landscape
Christen, M.T., Frank, P., Schaller, J., Llinas, M.
Biochemistry (2010), 49, 7131-7150, PubMed 20617841 , DOI 10.1021/bi100687f , Abstract

Related entities 1. hPgn rK3, : 1 : 6 : 450 entities Detail

Interaction partners 1. hPgn rK3, : 35 interactors Detail

Experiments performed 9 experiments Detail

NMR combined restraints 7 contents Detail